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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
HRP-conjugated HA Tag Monoclonal antibody
- 抗体英文名:
HA Antibody, HRP
- 靶点:
HRP-conjugated HA Tag Monoclonal
- 浓度:
1000 ug/ml
- 应用范围:
WB
- 宿主:
其它
- 级别:
科研
- 目录编号:
HRP-66006
- 适应物种:
HA Tag
- 供应商:
上海研卉生物科技有限公司
- 抗原来源:
其它
- 库存:
大量
- 标记物:
HRP
- 是否单克隆:
单克隆
- 亚型:
Mouse / IgG1
- 规格:
100ul
HRP-conjugated HA Tag Monoclonal antibody
宿主/亚型
Mouse / IgG1
种属反应性
recombinant protein
应用
WB
别名
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | HA Tag |
| 别名 | YPYDVPDYA |
| 计算分子量 | 1 kDa |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_3086576 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Protocol for anti-HA antibody Western Blotting
) 5) Incubate in primary antibody solution: I use PBS with 0.1% TWEEN-20 plus 5% BSA as the basic solution, but you can use TBS too. I dilute the HA antibody 1:5000, just to be sure you will see a signal. I haven't EVER seen appreciable background. I've
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
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