- ￥1550
- ImmuneChem
- CN0885
- 加拿大
- 2025年07月15日
- 其它
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
GST Antibody
- 宿主：
其它
- 库存：
大量
- 抗原来源：
其它
- 供应商：
上海研卉生物科技有限公司
- 是否单克隆：
0
- 规格：
100µg
GST Antibody 的详细介绍
- 类 型：Tag抗体
Product Description: This high affinity anti-GST-tag antibody is developed using GST peptide conjugated to KLH, the peptide sequence (EEHLYERDEGDKWR) is derived from common vector glutathione S transferase.Catalog Number Pack Size Price(CNY) CN0885 100 µg ￥1,550.00
Species: Rabbit
Formulation: 250 µg/mL antibody stored in PBS, pH 7, with 50% glycerol.
Storage & Stability: Store product at –20 ℃. Three years from date of shipping.
Immunogen: GST peptide EEHLYERDEGDKWR KLH conjugates.
Purification: Antigen-specific affinity chromatography.
Specificity: The antibody recognizes GST-tag proteins.
Application(s): ELISA (1:2500), WB (1:500-2000), IP (5 μg/sample), IF (5 μg/mL), IHC (5 μg/mL).
Scientific Description: GST is the glutathione S transferase, which is frequently used as affinity tag in recombinant proteins to facilitate the isolation, and localaiztion of the target proteins.
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文献和实验一、GST pull-down 基本知识1. GST pull-down 概念GST pull-down：是利用重组技术将探针蛋白与 GST 谷胱苷肽巯基转移酶融合，融合蛋白通过 GST 与固相化在球珠上的 GSH 谷胱甘肽结合。从而分离出能与融合蛋白相互作用的蛋白的技术。2. GST pull-down 研究对象二、GST pull-down 原理1. GST pull-down 结合原理2. GST pull-down 分离原理三、GST pull-down 流程1. 蛋白的抽提与纯化：①
mL PBS 1X 4- Centrifuge (1pulse at 500g) 5- Resuspend in 10 mL PBS 1X (stable for one month at 4 _ C) b) Extract preparation: 1- Transform BL21 or any appropriate bacterial strain with your GST-fused cDNA. Always use freshly transformed cells
with 1/2 volume of 50% GST beads at 4C rocking on the Nutator for 30-60 minutes. 50 ml conical Falcon tubes work well. Pour into a BioRad Column and drain lysate. Save 100 ul of depleted lysate. Load 2.5 ul for SDS-PAGE. Wash beads with 3 column volumes
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