Anti-Ras, clone RAS10; 100 μg

Anti-Ras, clone RAS10

05-516 is a recommended replacement for CBL751

The tumor oncoproteins HRas, KRas, and NRas are rated as Ras protoncogenes. Point mutations within Ras genes are frequently detected in human malignancies and in experimentally induced tumors in animals.

• GTP- and GDP-binding peptide B
• GTPase Hras
• Ha-Ras1 proto-oncoprotein
• Ras family small GTP binding protein H-Ras
• Transforming protein p2
• c-has/bas p21 protein
• c-ras-Ki-2 activated oncogene
• p19 H-RasIDX protein
• transformation gene: oncogene HAMSV
• v-Ha-ras Harvey rat sarcoma viral oncogene homolog

RAS10

This antibody recognizes p21H-, K- and N-Ras.

• ELISA
• Flow Cytometry
• Immunocytochemistry
• Immunohistochemistry
• Immunoprecipitation
• Western Blotting

Immunoprecipitation:
1-4 μg of this antibody has been reported to immunoprecipitate Ras.

Immunocytochemistry:
2.5 μg/mL of this antibody has been reported to show positive immunostaining for Ras in SW480 and Y1 cells.

Immunohistochemistry:
This antibody has been reported to detect Ras in frozen and paraffin embedded breast carcinoma sections.

• Human
• Rat
• Mouse

Proven to react with human, mouse and rat. Other species unknown.

IgG2aκ

Purified recombinant Ras. Clone RAS10.

21 kDa

Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Use 20 μg per lane for minigels

Purified mouse monoclonal IgG2ak in buffer containing PBS, pH 7.4, with 0.05% sodium azide and 30% glycerol. Liquid at -20°C.

Stable for 1 year at -20ºC from date of receipt.

• K-Ras
• Ki-Ras
• K-Ras2
• Kras-2
• p21B
• KRAS
• RASK2
• HRAS
• HA_RAS
• N-RAS
• H-RAS
• NRAS
• NRAS1
• ALPS4

Routinely evaluated by western blot on RIPA lysate from human A431 carcinoma cells, human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 or PC-12 cell lysates.

Western Blot Analysis:
0.05 - 0.5 μg/mL of this lot detected Ras in RIPA lysate from human A431 carcinoma cells. A previous lot detected Ras in human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 and PC- 12 cell lysates.

Signaling

• MAP Kinases
• G-proteins

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Upstate (Millipore)

1 mg/mL

Monoclonal Antibody

Purified

Mouse