3M 液体敷料 28ml 3346E （12瓶/箱）

Maxiprep of plasmid DNA from E. coli

worth of components for solution 2: 200μL 10% SDS, 100μL 4M NaOH, 1.7 mL autoclaved water 1.5mL Solution 3: 3M K+ , 5M acetate (3M potassium-acetate, 2M acetic acid -- glacial is 17M) 3.7mL isopropanol 1mL TE buffer 0.5mL 5M LiCl

Competent cells, calcium chloride method, E. coli, description

Reference: Dagert and Ehrlich (1979) Gene 6:23-28. Grow 5 ml overnight of the strain in 2 X YT in a standard container. Screw cap tight and lie flat on the shaker for good aeration. Dilute overnight culture

Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM

) isopropanol 70% ethanol 3M sodium acetate pH 5.2 Phase Lock GelTM (5 Prime, 3 Prime, Inc) 1. Grow E. coli culture overnight in rich broth