Human Nuclear Receptor Corepre

产品别名:KIAA1047 ELISA Kit, N-CoR ELISA Kit, TRAC1 ELISA Kit, hN-CoR ELISA Kit, Thyroid Hormone And Retinoic Acid Receptor Associated Corepressor 1 ELISA Kit

产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Receptor Corepressor 1(NCOR1).

应用说明：standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NCOR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NCOR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NCOR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NCOR1 in the samples is then determined by comparing the OD of the samples to the standard curve

检测时间:3.5h

UniProt ID:O75376

Note:For research use only.