- ￥4290
- biorbyt
- 3.13-200 ng/mL
- 0.96 ng/mL
- orb565259
- 英国
- 2026年01月20日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
biorbyt
- 检测范围：
3.13-200 ng/mL
- 检测方法：
Competitive
- 应用：
ELISA
- 适应物种：
All
- 样本：
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
0.96 ng/mL
- 规格：
48 T
产品描述:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Homovanillic Acid(HVA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Homovanillic Acid(HVA).
应用说明：standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVA in the samples is then determined by comparing the OD of the samples to the standard curve
检测时间:2h
Note:For research use only.
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文献和实验Neuroblastoma, in most cases, is characterized by increased production of catecholamines and their metabolites. Laboratory diagnosis and clinical follow-up include the measurement of urinary homovanillic acid (HVA) and vanillylmandelic acid
. ABTS Substrate Solution: 150 mg 2,2’-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (Sigma, Cat. No. A-1888) Add to 500 ml of 0.1M citric acid in ddH2 O Adjust pH to 4.35 with NaOH Aliquot 11 ml per vial and store at -200
of labeled moieties it contains. Antibodies can be labeled conveniently with iodine, enzymes, or biotin. General Protocol for the Sandwich ELISA method: Before the assay, both antibody preparations should be purified and one must be labeled.
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