Rat ATPase, Ca++ Transporting,

产品别名:ATP2B ELISA Kit, DAR ELISA Kit, SERCA2 ELISA Kit, Calcium pump 2 ELISA Kit, Endoplasmic reticulum class 1/2 Ca(2+) ATPase ELISA Kit, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle ELISA Kit

产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2(ATP2A2).

应用说明：standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ATP2A2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ATP2A2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ATP2A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ATP2A2 in the samples is then determined by comparing the OD of the samples to the standard curve

检测时间:3.5h

UniProt ID:P11507

Note:For research use only.