Cattle Fetuin A (FETUA) ELISA

产品别名:HSGa ELISA Kit, A2HS ELISA Kit, AHS ELISA Kit, FETUA ELISA Kit, HSG-A2 ELISA Kit, Fetuin-A ELISA Kit, Alpha 2-HS Glycoprotein ELISA Kit, Fetuin A ELISA Kit, Alpha-2-Z-Globulin ELISA Kit, Ba-alpha-2-Glycoprotein ELISA Kit

产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Heremans Schmid Glycoprotein(aHSG).

应用说明：standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle FETUA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle FETUA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle FETUA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle FETUA in the samples is then determined by comparing the OD of the samples to the standard curve

检测时间:3.5h

UniProt ID:P12763

Note:For research use only.