- ￥4680
- biorbyt
- 英国
- orb604609
- 2025年12月17日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期：
6个月
- 英文名：
Virus A27L protein
- 库存：
99
- 供应商：
biorbyt
- 规格：
20 ug
产品别名：A27L14 kDa fusion protein
应用笔记：Full Length
纯度：Greater than 85% as determined by SDS-PAGE.
生物来源：Vaccinia virus (strain Copenhagen) (VACV)
蛋白序列：MDGTLFPGDDDLAIPATEFFSTKADKKPEAKREAIVKADEDDNEETLKQRLTNLEKKITNVTTKFEQIEKCCKRNDEVLFRLENHAETLRAAMISLAKKIDVQTGRRPYE
分子量：19.6 kDa
表达区域：1-110aa
标签：N-terminal 10xHis-tagged and C-terminal Myc-tagged
防腐剂：If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源：E.coli
形态：Liquid or Lyophilized powder
Uniprot ID：P20535
Note：For research use only.
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文献和实验Isolation of Recombinant MVA Using F13L Selection
. This chapter describes the use of an efficient and easy-to-use selection system adapted for MVA. The system is based on the requirement of the viral gene F13L for efficient virus spread in cell culture, which results in a severe block in virus transmission
Virus Infection of Fibroblasts
containing 10% calf serum (prepare dilutions just before use): a. 1:10 (150 μl virus stock + 1,350 μl media) b. 1:100 (150 μl of 1:10 virus stock + 1,350 μl media) c. 1:1,000 (150 μl of 1:100 virus stock + 1,350 μl media) d. 1:10,000 (150 μl
applications. 10. For 12-well plate: (if in 6-well plate, duplicate all) Infect mouse cells: 400-800 μl virus + 800-400 μl fresh medium =1.2 ml total Infect human cells: 100-500 μl virus + 1100 μl-700 μl 11. Add 8-10 μg/ml polybrene, spin for 30 min
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