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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海泽叶生物科技有限公司
- 库存:
100
- 靶点:
见说明书
- 级别:
高
- 目录编号:
ZY92183
- 克隆性:
多克隆
- 抗原来源:
多抗制备
- 保质期:
2年
- 抗体英文名:
Fas-L
- 抗体名:
Fas-L
- 标记物:
无标记物
- 宿主:
小鼠
- 适应物种:
Human Other species not tested.
- 免疫原:
详见说明书
- 亚型:
Ig亚型IgG
- 形态:
液体或冻干粉
- 应用范围:
见说明书
- 保存条件:
-20°C
- 浓度:
0.5mg/ml
- 规格:
50ul/100ul /200ul
| 规格: | 50ul | 产品价格: | ¥1050.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1500.0 |
| 规格: | 200ul | 产品价格: | ¥2300.0 |
| Catalog No | ZY92183 |
|---|---|
| Species Reactivity | Human Other species not tested. |
| Immunogen | A peptide corresponding to middle region of human Fas-L protein. |
| GeneBank No. | NM_000639 |
| Alternative Names | ALPS1B; APT1LG1; APTL; CD178; CD95-L; CD95L; FASL; TNFSF6; TNLG1A; FASLG |
| Purification | Purified by protein A/G column. |
| Isotype | IgG |
| Formulation | Lyophilized power with 0.02% NaN3. Or dissolved in sterile H2O with 50% glycerol. |
| Applications | |
|---|---|
| ELISA | 1:20,000-1:80,000 |
| Immunohistochemistry | 1:100-1:500 |
| Western blot | 1:200-1:1,000 |
| For research use only. Optimal dilutions/concentrations should be determined by the final use. | |
| DATA | |||
|---|---|---|---|
|
|
|||
| Observed Molecular Weight | 31kd | ||
| PREPARATION AND STORAGE | |
|---|---|
| Reconstitution | If lyophilized, reconstitute in indicated volume of sterile H2O. For extended storage, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50%. |
| Storage | Shipped at ambient temperature or with ice bags if in liquid. Store immediately at-20°C upon receipt. Avoid repeating freeze-thaw cycles. |
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文献和实验相关专题 细胞凋亡专题 Fas/Fas L信号通路 最早被证明在细胞凋亡 中起着重要的作用。Fas是一种跨膜蛋白,属于肿瘤坏死因子受体超家族成员,它与FasL结合可以启动凋亡信号的转导引起细胞凋亡 。它的活化包括一系列步骤:首先配体诱导受体三聚体化,然后在细胞膜 上形成凋亡诱导复合物,这个复合物中包括带有死亡结构域的Fas相关蛋白FADD。Fas一旦和配体FasL结合,可通过Fas分子 启动致死性信号转导,最终
an important role in regulation of the immune system. In the method described, hepatoma MH134 (Fas− and FasL− ) is transfected with murine Fas and FasL cDNA. A single administration of monoclonal anti-Fas antibody efficiently suppresses the growth of F6b (MH134
Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography
Immobilized metal affinity chromatography (IMAC), introduced in 1975 (1 ), relies on the formation of coordinate bonds between metal ions immobilized on a suitable support and electron donor groups in proteins. A polyhistidine tag
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