This kit isolates untouched and highly purified B-CLL cells directly from human whole blood by fully immunomagnetic negative selection. The depletion cocktail in this kit does not contain antibodies against CD43, which can be expressed on malignant cells in chronic lymphocytic leukemia (CLL) samples.
This cell isolation kit targets non-B cells for removal based on specific surface antigens. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and can immediately be used in downstream applications such as nucleic acid extraction, flow cytometry or culture.
Advantages: • > 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation, or lysis
• Fast, easy-to-use and column-free
• Isolated cells are untouched
Components:
EasySep™ Direct Human B-CLL Cell Isolation Kit (Catalog #19664)
EasySep™ Direct Human B-CLL Cell Isolation Cocktail, 2 x 2.5 mL