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文献和实验Immunoprecipitation of Proteins
when manipulating agarose beads to avoid disruption of the beads. 6.Pre-clear the cell lysate by adding 100 microliters of either protein A or G agarose/sepharose bead slurry (50%)per 1 ml of cell lysate and incubating at 4? for 10 minutes on a rocker or orbital
1.Wash adherent cells twice in the dish or flask with ice-cold PBS and drain off PBS.Wash non-adherent cells in PBS and centrifuge at 800 to 1000 rpm in a table-top centrifuge for 5 minutes to pellet the cells. 2.Add ice-cold to cells (1 ml per 107
1. Wash adherent cells twice in the dish or flask with ice-cold PBS and drain off PBS. Wash non-adherent cells in PBS and centrifuge at 800 to 1000 rpm in a table-top centrifuge for 5 minutes to pellet the cells. 2. Add ice-cold modified RIPA
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