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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Thyrotropin subunit beta (TSHB)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥10872.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2514.0 |
| 规格: | 20μg | 产品价格: | ¥1344.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
TSHBUniprot No.:
P01222别名:
Thyroid-stimulating hormone subunit beta ;TSH-B ;TSH-betaThyrotropin beta chainINN: Thyrotropin alfa种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
28.9 kDa表达区域:
21-132aa氨基酸序列:
FCIPTEYTMHIERRECAYCLTINTTICAGYCMTRDINGKLFLPKYALSQDVCTYRDFIYRTVEIPGCPLHVAPYFSYPVALSCKCGKCNTDYSDCIHEAIKTNYCTKPQKSY蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Indispensable for the control of thyroid structure and metabolism.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
Signal TransductionReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
A novel thyroid stimulating hormone beta-subunit isoform in human pituitary, peripheral blood leukocytes, and thyroid.Schaefer J.S., Klein J.R.Gen. Comp. Endocrinol. 162:241-244(2009)Function:
Indispensable for the control of thyroid structure and metabolism.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
A novel thyroid stimulating hormone beta-subunit isoform in human pituitary, peripheral blood leukocytes, and thyroid.Schaefer J.S., Klein J.R.Gen. Comp. Endocrinol. 162:241-244(2009)
相关实验
剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择: 尿素和盐酸胍属中强度变性剂,易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用,所需浓度尿素8-10M,盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱,溶解度为70-90%,尿素在作用时间较长或温度较高时会裂解形成氰酸盐,对重组蛋白质的氨
技术资料暂无技术资料 索取技术资料
文献支持
TSHB/TSHB蛋白Recombinant Human Thyrotropin subunit beta (TSHB)重组蛋白Thyroid-stimulating hormone subunit beta ;TSH-B ;TSH-betaThyrotropin beta chainINN: Thyrotropin alfa蛋白
¥1344 - 10872





