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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Bacillus subtilis Malate dehydrogenase (mdh)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Bacillus subtilis Malate dehydrogenase (mdh)品名简称:
Recombinant Bacillus subtilis mdh protein货号:
CSB-EP013623BRJ规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
mdhUniprot No.:
P49814别名:
Vegetative protein 69 ;VEG69种属:
Bacillus subtilis (strain 168)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
37.5 kDa表达区域:
2-312aa氨基酸序列:
GNTRKKVSVIGAGFTGATTAFLIAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDAKITGTSNYEDTAGSDIVVITAGIARKPGMSRDDLVSTNEKIMRSVTQEIVKYSPDSIIVVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTFVAEELNLSVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLETLIPKERIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTEMVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIVGGNGLEQIIELELTDYERAQLNKSVESVKNVMKVLS蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Catalyzes the reversible oxidation of malate to oxaloacetate.功能2:
Catalyzes the reversible oxidation of malate to oxaloacetate.文献:
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R., Mann M.Mol. Cell. Proteomics 6:697-707(2007)研究领域:
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文献和实验High‐Throughput Screening for Protein‐Protein Interactions Using Yeast Two‐Hybrid Arrays
, and most of them are known PHO85 interactors; CLG1 is a cyclin‐like protein; CDC36 is a transcription factor; YPL229W is a protein of unknown function; SOR1 is sorbitol dehydrogenase; and YDL246C is a SOR1‐related protein. Most of these preys are either known or highly
polyacrylamide gel electrophoresis (SDS-PAGE) to locate the standards and the test protein. Figure 1 shows an example determining the sedimentation coefficient of the structural maintenance of chromosome (SMC) protein from Bacillus subtilis
Survey of Protein Engineering Strategies
Bartsch, S., Kourist, R. and Bornscheuer, U.T. 2008. Complete inversion of enantioselectivity towards acetylated tertiary alcohols by a double mutant of a Bacillus subtilis esterase. Angew. Chem. Int. Ed. 47:1508‐1511
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