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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃/-80℃
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human L-serine dehydratase/L-threonine deaminase (SDS)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100ug/20ug
| 规格: | 1mg | 产品价格: | ¥12372.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥2880.0 |
| 规格: | 20ug | 产品价格: | ¥1536.0 |
Research Areas
NeuroscienceUniprot ID
P20132Gene Names
SDSAlternative Name(s)
(SDH)(L-serine deaminase)(L-threonine dehydratase)(TDH)Lead Time
3-7 business daysOrganism
Homo sapiens (Human)Source
E.coliExpression Region
1-328aaProtein Length
Full LengthTag Info
N-terminal 10xHis-tagged and C-terminal Myc-taggedTarget Protein Sequence
MMSGEPLHVKTPIRDSMALSKMAGTSVYLKMDSAQPSGSFKIRGIGHFCKRWAKQGCAHFVCSSAGNAGMAAAYAARQLGVPATIVVPSTTPALTIERLKNEGATVKVVGELLDEAFELAKALAKNNPGWVYIPPFDDPLIWEGHASIVKELKETLWEKPGAIALSVGGGGLLCGVVQGLQEVGWGDVPVIAMETFGAHSFHAATTAGKLVSLPKITSVAKALGVKTVGAQALKLFQEHPIFSEVISDQEAVAAIEKFVDDEKILVEPACGAALAAVYSHVIQKLQLEGNLRTPLPSLVVIVCGGSNISLAQLRALKEQLGMTNRLPKMW
42.1 kDaPurity
Greater than 90% as determined by SDS-PAGE.Endotoxin
Not test.Biological_Activity
/Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.Relevance
/Function
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文献和实验完整蛋白质从 SDS-PAGE 胶中的电洗脱及其 MALDI-TOF MS 分析
1. 前言 基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS) 已经被广泛应用于生 物科学中。结合十二烷基磺酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE ) 和酶解消化,它提供了一种通过肽质量指纹谱(PMF ) 的快速、灵敏和准确鉴定蛋白质的方法通常 PMF 给出 10%~40% 的序列覆盖率。这样的覆盖率对于蛋白质鉴定来说已经足够,但它可能还不足以用来区分高度相似的蛋白质异构体。蛋白质异构体/同工酶广泛存在,它们通过 mRNA 的可变剪切或多基因家族生成。虽然它们在细胞中催化
膜蛋白的分离、鉴定及其功能分析—GFC/IEC/SDS-PAGE 和 MALDI-TOF-MS 方法
烯酰胺凝胶电泳可有效分离膜镶嵌蛋白质。 下面要介绍的方法是以离子交换层析替代 2D-GE 电泳中 IEF,如果样品量充足,还可以辅以凝胶过滤色谱层析 [7] 。最终用 SDS-PAGE 电泳进一步分离膜镶嵌蛋白质。经过上述这些分离过程,许多膜镶嵌蛋白的胶内酶解和 MALDI-TOF/MS 分析获得了成功。 这个方法的目标之一是在一个单一的实验构架内,分离膜组分中不同类别的蛋白质,包括大部分的疏水、酸性和碱性蛋白。它们可通过染色胶和 MALDI-TOF/MS 蛋白质指纹谱进行蛋白质分析。本章节介绍
Blot)的第一步,蛋白质修饰及免疫沉淀蛋白的鉴定等。 【器材与试剂】 1. 微型凝胶电泳装置 (如:Bio-Rad Mini-Protein Ⅲ型电泳仪),电泳电源,100℃沸水浴装置,Eppendorf管,微量注射器,带盖塑料染色盒,摇床。 2. 制胶试剂 丙烯酰胺(电泳级),N,N'-甲叉双丙烯酰胺,Tris碱,SDS,TEMED,过硫酸铵,DTT或α-巯基乙醇,甘油,溴酚蓝,甘氨酸,盐酸。 3. 染色/脱色试剂 考马斯亮蓝R-250,甲醇,冰醋酸 4. 储存液
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