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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃,避光
- 英文名:
Sulfo-Cy3-E-dUTP
- 库存:
999
- 供应商:
Biorigin
- 规格:
25nmole
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文献和实验ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5: A. MATERIALS RNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat # 18064-014) QIAquick PCR Purification Kit (Qiagen; Cat # 28106)
How Do I make an Antibody against a Peptides
If your peptide is synthesized with a terminal cysteine residue (i.e., a free sulfhydryl group, -SH), you can use any maleimide-activated carrier protein to create an immunogen. One-step chemical reactions are easy with Pierce Sulfo-SMCC Pre
Revised amino-allyl labeling protocol
at RT in the dark. Mix every 15 minutes. E. Quenching and Cleanup Before combining Cy3 and Cy5 samples for hybridization, unreactive NHS-ester Cy dye must be quenched to prevent cross coupling. Add 4.5 m L 4M hydroxylamine (Sigma







