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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃
- 英文名:
5,5’-Dimethyl BAPTA, tetrasodium salt
- 库存:
999
- 供应商:
Biorigin
- 规格:
5 mg
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文献和实验Growing feeder-independent embryonic stem cells§
before use. Trypsin solution: Add 100 mg of EDTA tetrasodium salt (Sigma) to 500 ml of PBS. Filter sterlize and add 10 ml (for 1×) or 20 ml (for 2×) of 2.5% trypsin solution (Gibco-BRL) and 5 ml of chicken serum (Gibco-BRL). Store in 20 ml aliquots
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
0.2 ml 0.1M Tris buffer pH 8.2 9.8 ml Levamisole (Sigma L 9756) 2.4 mg Fast Red TR salt (Sigma F 1500) 10 mg Dissolve naphthol-AS-MX phosphatase, free acid in N,N-dimethyl formamide. Add the Tris buffer. Dissolve and Fast Red TR
MTT Growth Assays in Ovarian Cancer
of this assay is in the screening of large numbers of samples. The MTT assay, a quantitative colorimetric assay is based on the living cell’s ability to reduce the tetrazolium salt MTT, a pale yellow substrate to a dark-blue formazan product. The mitochondrial







