/gap重组蛋白(GAPDH)(NAD-dependent

英文名称：

Recombinant Lactobacillus delbrueckii subsp. bulgaricus Glyceraldehyde-3-phosphate dehydrogenase (gap)

品名简称：

Recombinant Lactobacillus delbrueckii subsp. bulgaricus gap protein

货号：

CSB-EP009232LMM

规格:

20μg

价格：

2328

LC-MS/Western Blot：

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SDS-PAGE：

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

纯度：

Greater than 90% as determined by SDS-PAGE.

内毒素：

Not test

生物活性：

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基因名：

gap

Uniprot No.：

O32755

别名：

(GAPDH)(NAD-dependent glyceraldehyde-3-phosphate dehydrogenase)

种属：

Lactobacillus delbrueckii subsp. bulgaricus

蛋白长度：

Full Length

来源：

E.coli

分子量：

44.0 kDa

表达区域：

1-338aa

氨基酸序列：

MTVKIGINGFGRIGRLAFRRIMDLGEETKDIEVVAINDLTTPAMLAHLLKYDSTHGTFDHEVSATEDSLVVDGKKYRVYAEPQAQNIPWVKNDGVDFVLECTGFYTSKAKSQAHLDAGAKRVLISAPAGNDLKTIVYSVNQDTLTADDTIVSAGSCTTNSLAPMANALNKEFGIQVGTMTTIHAYTATQKVLDGPDRGNNFRNARAAAENIIPHSTGAAKAIGLVLPELNGKLDGHAQRVPVKDGSETELVTILDKKVTAEEVNAAMKKYESPSFAYNADQIVSTDVLGMTAGSIFDPTQTQVITAGDKQLVKTVAWYDNEYSFTCQMVRTLLHFATL

蛋白标签：

N-terminal 10xHis-tagged and C-terminal Myc-tagged

产品提供形式：

Liquid or Lyophilized powder

缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

储存条件：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

货期：

3-7 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能1：

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.

功能2：

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文献：

"An operon encoding three glycolytic enzymes in Lactobacillus delbrueckii subsp. bulgaricus: glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase and triosephosphate isomerase." Branny P., Delatorre F., Garel J.R. Microbiology 144:905-914(1998)

研究领域：

Others

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