5'-核苷酸酶活性测定试剂盒/5'-核苷酸酶活性测定试剂盒/

产品名称：5'-核苷酸酶活性测定试剂盒/5'-核苷酸酶活性测定试剂盒/5'-核苷酸酶活性测定试剂盒-5’-Nucleotidase Activity Assay Kit /5’-Nucleotidase Activity Assay Kit /5’-Nucleotidase Activity Assay Kit

产品货号：K992-100

产品规格：100assays

背景资料：待更新

保存建议：5'-核苷酸酶活性测定试剂盒/5'-核苷酸酶活性测定试剂盒/5'-核苷酸酶活性测定试剂盒-5’-Nucleotidase Activity Assay Kit /5’-Nucleotidase Activity Assay Kit /5’-Nucleotidase Activity Assay Kit -20°C

产品描述：5’-Nucleotidase (5’-NT), also known at CD73 (EC 3.1.3.5) is an enzyme located in the plasma membrane. It converts extracellular nucleotides like 5’-AMP to their corresponding nucleosides, through phosphorylitic cleavage. This conversion facilitates uptake of the nucleosides through nucleoside receptors into the cell, where they can again be phosphorylated to generate nucleotides and contribute to the nucleotide pool, inside the cell. 5’-NT levels are elevated in hepatic diseases such as viral hepatitis, alcoholic liver disease and cirrhosis. BioVision’s 5’-Nucleotidase Activity Kit is a simple two-step end point assay that relies on the Berthelot’s test for quantification of ammonia. In this assay, the action of 5’-nucleotidase on the substrate generates a product, which releases ammonia in presence of the converter. Developer I and II are then used to quantify the released ammonia through increase in absorbance at 670 nm. This assay can detect as low as 0.2 mU of 5’-NT. Since non-specific enzymes like alkaline phosphatase can give a positive signal in this assay, 5’-NT inhibitor may be used to completely inhibit 5’-nucleotidase and distinguish from the signal from non-specific enzymes. The assay kit also includes 5’- Nucleotidase (5’-NT) enzyme for use as positive control

点击：5'-核苷酸酶活性测定试剂盒/5'-核苷酸酶活性测定试剂盒/5'-核苷酸酶活性测定试剂盒-5’-Nucleotidase Activity Assay Kit /5’-Nucleotidase Activity Assay Kit /5’-Nucleotidase Activity Assay Kit 查看更详细产品说明，更多应用、储存、价格、货期等信息请致电垂询BioVision中国区域授权总代理艾美捷科技有限公司。更多特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看每日生物评论网站。

BioVision的公司总部在美丽的旧金山湾地区，它是全球生命科学公司的领路人.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品，以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理，艾美捷与BioVision一起为研究者们提供最好、崭新的细胞生物学研究工具而不断扩大产品及服务质量.

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关键词：细胞分析试剂盒，5'-核苷酸酶活性测定试剂盒/5'-核苷酸酶活性测定试剂盒/5'-核苷酸酶活性测定试剂盒，待更新