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ARF5/ARF5蛋白Recombinant Human A

DP-ribosylation factor 5 (ARF5)重组蛋白ADP ribosylation factor 5; ADP-ribosylation factor 5; ARF 5; Arf5; ARF5_HUMAN蛋白
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  • ¥1344 - 10872
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  • CN
  • CSB-RP009054h
  • 2025年07月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Human ADP-ribosylation factor 5 (ARF5)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      1mg/100μg/20μg

    规格:1mg产品价格:¥10872.0
    规格:100μg产品价格:¥2514.0
    规格:20μg产品价格:¥1344.0

    纯度:

    Greater than 90% as determined by SDS-PAGE.

    基因名:

    ARF5

    Uniprot No.:

    P84085

    别名:

    ADP ribosylation factor 5; ADP-ribosylation factor 5; ARF 5; Arf5; ARF5_HUMAN

    种属:

    Homo sapiens (Human)

    蛋白长度:

    Full Length of Mature Protein

    来源:

    E.coli

    分子量:

    47.4 kDa

    表达区域:

    2-180aa

    氨基酸序列:

    GLTVSALFSRIFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR

    蛋白标签:

    N-terminal GST-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    货期:

    13-23 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能:

    GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

    内毒素:

    Not test.

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    LC-MS Image Description/Western Blot:

    /

    Product types:

    Developed Protein

    Biological_Activity:

    /

    Research Areas:

    Transport

    Reconstitution:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    Reference:

    Molecular identification of ADP-ribosylation factor mRNAs and their expression in mammalian cells.Tsuchiya M., Price S.R., Tsai S.-C., Moss J., Vaughan M.J. Biol. Chem. 266:2772-2777(1991)

    Function:

    GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

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    图标文献和实验
    该产品被引用文献
    Molecular identification of ADP-ribosylation factor mRNAs and their expression in mammalian cells.Tsuchiya M., Price S.R., Tsai S.-C., Moss J., Vaughan M.J. Biol. Chem. 266:2772-2777(1991)
    相关实验
    • Preparation of Myristoylated Arf1 and Arf6 Proteins

      ADP-ribosylation factor (Arf) proteins were first identified as cofactors for cholera toxin-catalyzed ADP-ribosylation of the heterotrimeric G-protein Gs. Subsequent cloning led to the discovery that Arfs were part of a group of GTP-binding

    • 蛋白复性(二)-基因重组蛋白质的体外复性

      X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏蛋白

    • 仅扩增全长cDNA末端的高级RACE方法

      ) M26066 2.8 +16 ADP-ribosylation factor 4 (ARF4) M36341.1 1.5 +116 Isoleucil-tRNA synthetase U04953

    图标技术资料

    暂无技术资料 索取技术资料

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    文献支持
    ARF5/ARF5蛋白Recombinant Human ADP-ribosylation factor 5 (ARF5)重组蛋白ADP ribosylation factor 5; ADP-ribosylation factor 5; ARF 5; Arf5; ARF5_HUMAN蛋白
    ¥1344 - 10872