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RbcL | Rubisco large subunit,

form I, HRP-conjugated (40 µg)
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  • 询价
  • Agrisera
  • AS03 037-HRP
  • 瑞典
  • 2025年11月14日
  • ELISA (ELISA), Western blot (WB)
  • Rabbit
  • Arabidopsis thaliana, Apium graveolens, Artemisia annua, Baculogypsina sphaerulata (benthic foraminifer), Bienertia sinuspersici, Cicer arietinum, Chlamydomonas raudensis, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Cyanophora paradoxa, C
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      RbcL | Rubisco large subunit, form I, HRP-conjugated (40 µg)

    • 抗体英文名

      RbcL | Rubisco large subunit, form I, HRP-conjugated (40 µg)

    • 靶点

      Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each prese

    • 应用范围

      ELISA (ELISA), Western blot (WB)

    • 宿主

      Rabbit

    • 适应物种

      Arabidopsis thaliana, Apium graveolens, Artemisia annua, Baculogypsina sphaerulata (benthic foraminifer), Bienertia sinuspersici, Cicer arietinum, Chlamydomonas raudensis, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Cyanophora paradoxa, C

    • 抗原来源

      O03042 , P05698 , P0C510 , P00877

    • 级别

      Affinity purified serum

    • 供应商

      Agrisera AB

    • 克隆性

      单克隆

    • 保存条件

      Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

    • 形态

      Liquid in PBS pH 7.4, conjugated to HRP

    • 免疫原

      KLH-conjugated synthetic peptide conserved across all known plant, algal and (cyano)bacterial RbcL protein sequences (form I L8S8 and form II L2), including Arabidopsis thaliana AtCg00490, Hordeum vulgare P05698, Oryza sativa P0C510, Chlamydomonas reinhar

    • 规格

      40 µg

    Anti-RbcL can be used as a cellular [compartment marker] of plastid stroma (cytoplasm in cyanobacteria) and detects RbcL protein from 31.25 fmoles. As both forms (I and II) are detected it is suitable for work with samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brown algae) as well as higher plants. This antibody together with Agrisera Rubisco protein standard is very suitable to quantify Rubisco in plant and algal samples.
    Immunogen:

    KLH-conjugated synthetic peptide conserved across all known plant, algal and (cyano)bacterial RbcL protein sequences (form I L8S8 and form II L2), including Arabidopsis thaliana AtCg00490,Hordeum vulgare P05698Oryza sativa P0C510Chlamydomonas reinhardtii P00877Synechococcus PCC 7920 A5CKC5

    Host: Rabbit 
    Clonality: Polyclonal 
    Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP. 
    Format: Liquid 
    Quantity: 40 µg 
    Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. 
    Tested applications: ELISA (ELISA), Western blot (WB) 
    Recommended dilution: 1 : 5000 (ELISA), 1 : 10 000-25 000 (WB) 
    Expected | apparent MW:

    52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)

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    图标文献和实验
    相关实验
    • Far-Western Blotting

      and water to remove SDS from the gel and allow the prey protein to renature. The gel is then incubated with the bait protein (usually in the pure form). If the bait protein is biotinylated, it is subsequently detected with streptavidin-HRP and a highly

    • Far-Western Blotting

      with streptavidin-HRP and a highly sensitive formulation of Pierce’s patented SuperSignal Chemiluminescent Substrate. If the bait protein is fusion-tagged, detection is with an anti-tag HRP-conjugated antibody and the chemiluminescent substrate. The same controls

    • Identification and Analysis of Multiprotein Complexes Through Chemical Crosslinking

      buffer (see recipe ) ∼80 µg His6‐GST‐TEVcs‐bait protein ∼20 µg His6‐GST‐TEVcs (control

    图标技术资料

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