
RbcL | Rubisco large subunit,
form I, HRP-conjugated (40 µg)- 询价
- Agrisera
- AS03 037-HRP
- 瑞典
- 2025年11月14日
- ELISA (ELISA), Western blot (WB)
- Rabbit
- Arabidopsis thaliana, Apium graveolens, Artemisia annua, Baculogypsina sphaerulata (benthic foraminifer), Bienertia sinuspersici, Cicer arietinum, Chlamydomonas raudensis, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Cyanophora paradoxa, C
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
RbcL | Rubisco large subunit, form I, HRP-conjugated (40 µg)
- 抗体英文名:
RbcL | Rubisco large subunit, form I, HRP-conjugated (40 µg)
- 靶点:
Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each prese
- 应用范围:
ELISA (ELISA), Western blot (WB)
- 宿主:
Rabbit
- 适应物种:
Arabidopsis thaliana, Apium graveolens, Artemisia annua, Baculogypsina sphaerulata (benthic foraminifer), Bienertia sinuspersici, Cicer arietinum, Chlamydomonas raudensis, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Cyanophora paradoxa, C
- 抗原来源:
O03042 , P05698 , P0C510 , P00877
- 级别:
Affinity purified serum
- 供应商:
Agrisera AB
- 克隆性:
单克隆
- 保存条件:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
- 形态:
Liquid in PBS pH 7.4, conjugated to HRP
- 免疫原:
KLH-conjugated synthetic peptide conserved across all known plant, algal and (cyano)bacterial RbcL protein sequences (form I L8S8 and form II L2), including Arabidopsis thaliana AtCg00490, Hordeum vulgare P05698, Oryza sativa P0C510, Chlamydomonas reinhar
- 规格:
40 µg
| Immunogen: | KLH-conjugated synthetic peptide conserved across all known plant, algal and (cyano)bacterial RbcL protein sequences (form I L8S8 and form II L2), including Arabidopsis thaliana AtCg00490,Hordeum vulgare P05698, Oryza sativa P0C510, Chlamydomonas reinhardtii P00877, Synechococcus PCC 7920 A5CKC5 |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Purity: | Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP. |
| Format: | Liquid |
| Quantity: | 40 µg |
| Storage: | Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. |
| Tested applications: | ELISA (ELISA), Western blot (WB) |
| Recommended dilution: | 1 : 5000 (ELISA), 1 : 10 000-25 000 (WB) |
| Expected | apparent MW: | 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii) |
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文献和实验and water to remove SDS from the gel and allow the prey protein to renature. The gel is then incubated with the bait protein (usually in the pure form). If the bait protein is biotinylated, it is subsequently detected with streptavidin-HRP and a highly
with streptavidin-HRP and a highly sensitive formulation of Pierce’s patented SuperSignal Chemiluminescent Substrate. If the bait protein is fusion-tagged, detection is with an anti-tag HRP-conjugated antibody and the chemiluminescent substrate. The same controls
Identification and Analysis of Multiprotein Complexes Through Chemical Crosslinking
buffer (see recipe ) ∼80 µg His6‐GST‐TEVcs‐bait protein ∼20 µg His6‐GST‐TEVcs (control





