- 询价
- Agrisera
- 瑞典
- AS14 2820
- 2025年11月14日
- 1 : 1000 (WB)
- Rabbit
- Chlamydomonas reinhardtii
企业认证
相关产品推荐更多 >
Goat anti-Mouse IgG1 (subclass specific), HRP conjugated
询价Goat anti-Mouse IgG (H&L), Biotin conjugated, min, cross-reactivity to human IgG/serum
询价Chicken anti-Mouse IgG (H&L), Affinity purified, Unconjugated
询价
Anti-SPS | Sucrose phosphate synthase, global, Biotin-conjugated (40 µg)
询价Anti-His-tag | 3xHis (polyclonal) - DyLight® 650 conjugated (40 µg)
询价
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
recombinant Chlamydomonas reinhardii RBR40 UniProt: Q6EMK7, AY124882
- 形态：
Lyophilized
- 保存条件：
Store lyophilized/reconstituted at -20°C; once rec
- 克隆性：
Polyclonal
- 标记物：
Schwarz at al.. (2007). Synthesis of the D2 protein of photosystem II in Chlamydomonas is controlled by a high molecular mass complex containing the RNA stabilization factor Nac2 and the translational activator RBP40. Plant Cell, 19, 3627–3639.
- 适应物种：
Chlamydomonas reinhardtii
- 级别：
分子生物学级
- 供应商：
Agrisera
- 宿主：
Rabbit
- 应用范围：
1 : 1000 (WB)
- 靶点：
RBP40 (38 kDa RNA-binding protein) binds to the psbD 5'UTR in a Nac2-dependent fashion both in vitro and in vivo and specifically affects the initiation of D2 synthesis. Alternative name: Chloroplast-targeted RNA-binding protein.
- 抗体名：
RBP40 | 38 kDa RNA-binding protein
- 规格：
50 µl
application example
7.5 µg of total protein from Chlamydomonas reinhardtii extracted with lysis buffer (120mM KCl, 20 mM Tricine pH 7.8, 0.4 mM EDTA, 5 mM ß-Mercaptoethanol and 1% Triton-X100), were separated on a 12 % SDS-PAGE and blotted 1h to nitrocellulose using semi-dry transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for ON at 4C with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Sigma, A9169) diluted to 1:10000 in 5% milk in TBS-T for 1h at RT with agitation. The blot was rinsed briefly once, then washed 4 times for 10 min and developed with ECL according to the manufacturer's instructions. Exposure time was 4 seconds.
Courtesy of Dr. Jörg Nickelsen, LMU München, Germany
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验PAIR Technology: Exon-Specific RNA-Binding Protein Isolation in Live Cells
RNA-binding proteins (RBPs) are fundamental regulatory proteins for all forms of transcriptional and posttranscriptional control of gene expression. However, isolating RBPs is technically challenging for investigators
Advances in RIP-Chip Analysis: RNA-Binding Protein Immunoprecipitation-Microarray Profiling
complexes from cellular extracts and identifying the associated messages using genome-scale, microarray technology, a method called ribonomics or RNA-binding protein immunoprecipitation-microarray (Chip) profiling or RIP-Chip . The use of the RIP-Chip
Expression and Mutagenesis of Retinol-Binding Protein
Vitamin A is transported in the plasma as retinol bound to a carrier protein, called retinol-binding protein (RBP), which itself forms a complex with the thyroxine-binding protein, known as transthyretin (TTR). This complex exists in equilibrium
