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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated peptide conserved in Major carboxysome shell protein 1A, UniProt: P45689, Major carboxysome shell protein 1B UniProt: P45690, Major carboxysome shell protein 1C, UniProt: P45688
- 形态:
Lyophilized
- 保存条件:
Store lyophilized/reconstituted at -20°C; once rec
- 克隆性:
Polyclonal
- 标记物:
Long et al. (2018). Carboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts. Nat Commun. 2018 Sep 3;9(1):3570. doi: 10.1038/s41467-018-06044-0.
- 适应物种:
Halothiobacillus neapolitanus (strain ATCC 23641 / c2) (Thiobacillus neapolitanus)
- 级别:
分子生物学级
- 供应商:
Agrisera
- 宿主:
Rabbit
- 应用范围:
1 : 5000 (WB)
- 靶点:
Major carboxysome shell protein is involved in the formation of the carboxysome, polyhedral includion sequestering Rubisco.
- 抗体名:
CsoS1A/B/C | Major carboxysome shell protein 1A, AB, 1C
- 规格:
50 µg
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文献和实验Immunohistochemistry: Fluorescence Protocol 2.0
. Sample Preparation and Fixation Harvest cells and wash twice in V-bottomed tubes with cold Wash Buffer by centrifugation (400 x g for 5 minutes) at 4°C to remove extracellular cytokines. Add 0.5 mL of the Fixation Buffer
Immunohistochemisty-Fluorescence Protocol
, store at 4°C in the dark; prepare working dilution just prior to fixation Wash Buffer Earls Buffered Salt Solution (EBSS) (Gibco BRL) Wash Buffer-Saponin EBSS with 0.1 (w/v) Saponin (Sigma Catalog # S4521) and adjust pH to 7.2-7.4 with NaOH
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
sec. break x 3 times, 20% amplitude with a small tip). Now the length of your chromatin DNA should be 200-1000 bp. (13) Centrifuge for 20 min at 14000 rpm, 4°C. Recover supernatant. This is the soluble sheared chromatin fraction. This can be stored
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