相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
AGO2 | Argonaute 2
- 抗体英文名:
AGO2 | Argonaute 2
- 靶点:
AGO2 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). AGO2 is probably involved in antiviral RNA silencing.
- 应用范围:
Immunoprecipitation (IP), Western blot (WB)
- 宿主:
Rabbit
- 适应物种:
Arabidopsis thaliana
- 抗原来源:
Q9SHF3
- 级别:
Affinity purified serum in PBS, pH 7.4
- 供应商:
Agrisera AB
- 克隆性:
单克隆
- 保存条件:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the ca
- 形态:
Lyophilized
- 免疫原:
KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana AGO2 protein, UniProt: Q9SHF3, TAIR: AT1G31280
- 规格:
50 µg
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验MicroRNA Northern Blotting, Precursor Cloning, and Ago2-Improved RNA Interference
about their role in cancer. Besides Drosha and Dicer, Argonaute proteins are key players in miRNA processing. In addition to their role as components of the RNA-induced silencing complex (RISC) executing target silencing, Argonautes mediate posttranscriptional
使用抗Argonaute抗体进行microRNA研究的方法介绍
表达最多的是Ago2,它具有Slicer活性,可切割其靶RNA,在microRNA调控路径中具有重要意义。针对这种Ago蛋白,利用抗体,经RISC免疫沉淀,即microRNA与靶mRNA共沉淀,由此可回收microRNA。 图1. microRNA的克隆和作用原理 本实验组用Argonaute蛋白抗体,纯化并克隆microRNA和靶mRNA(图2)。使用该技术,可综合分析存在于细胞、组织等RISC中的microRNA和mRNA。以下将简单介绍各种技术的使用方法、分析实例
)基和氨基的反应 在RNA免疫沉淀法里的应用 其次,我们介绍一下在RNA免疫沉淀法里应用本方法制作的抗体固相化磁珠的例子。在二氧化硅磁珠里使用了固相化抗Argonaute抗体的磁珠,通过RNA免疫沉淀法可得到结合到Argonaute蛋白质里的microRNA。实验中固相化了抗人Ago2抗体磁珠的人Ago2,从人细胞株的细胞裂解液中纯化结合在Ago2里的microRNA。结果可从各种人细胞株里得到高纯度的microRNA(图2)。 图2、抗体固相化磁珠在RNA免疫
技术资料暂无技术资料 索取技术资料





