Goat anti-Mouse IgG (H&L), HRP
conjugated, min. cross-reactivity to bovine, goat,human, rabbit, rat IgG- 询价
- Agrisera
- 瑞典
- AS10 964
- 2025年11月17日
- The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1 : 20-1 : 2000 depending upon the application (immunolocalization or western blot).
- Goat
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
Lyophilized
- 保存条件：
Store lyophilized material at 2-8°C.For long time
- 克隆性：
Polyclonal
- 级别：
分子生物学级
- 供应商：
Agrisera
- 宿主：
Goat
- 应用范围：
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1 : 20-1 : 2000 depending upon the application (immunolocalization or western blot).
- 靶点：
Goat anti-mouse IgG (H&L) is a secondary antibody HRP conjugated which binds to all mouse IgGs in immunological assays. It is specially adsorbed against bovine,goat,human,rabbit,rat IgG.
- 抗体名：
Goat anti-Mouse IgG (H&L), HRP conjugated, min. cross-reactivity to bovine, goat,human, rabbit, rat IgG
- 规格：
1 mg
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文献和实验我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
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