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- 详细信息
- 文献和实验
- 技术资料
- 形态:
Immunogen affinity purified IgG.
- 保存条件:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
- 克隆性:
Polyclonal
- 供应商:
Agrisera
- 宿主:
Donkey
- 靶点:
Donkey anti-Rat IgG (H&L), Biotin conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, sheep IgG
- 抗体英文名:
Donkey anti-Rat IgG (H&L), Biotin conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, sheep IgG
- 抗体名:
Donkey anti-Rat IgG (H&L), Biotin conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, sheep IgG
- 规格:
1 mg
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
without washing and apply the primary antibody, in a moist chamber, at RT for 1-18 hr. 5- Wash twice in TBS 0.05M pH7.5 + 0.01% Tween 20. 6- add the biotin-conjugated secondary antibody (50 to 100 µl) and incubate for 45 min. The secondary antibody
20. 7bis- block endogenous peroxidase by incubating in 0.1%NaN 3 and 0.3% H 2 O 2 for 30 min. Wash thrice. 8- add the HRP-conjugated avidin (50 to 100 µl, dilution 1:300 -500 in nTBS-Tween
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