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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
native peroxidase isolated and purified from horseradish
- 形态:
Lyophilized in phosphate buffer saline
- 保存条件:
Store lyophilized/reconstituted at -20°C; once rec
- 克隆性:
Polyclonal
- 标记物:
Rup et al. (2017). Immunogenicity of glycans on biotherapeutic drugs produced in plant expression systems-The taliglucerase alfa story. PLoS One. 2017 Oct 31;12(10):e0186211. doi: 10.1371/journal.pone.0186211.
- 适应物种:
Armoracia rusticana
- 级别:
分子生物学级
- 供应商:
Agrisera
- 宿主:
Rabbit
- 应用范围:
1 : 1000-1 : 10 000 (ELISA), (IF), (IHC), (WB)
- 靶点:
Horseradish peroxidase removes hydrogen peroxide, acts in oxidation of toxic reductants, biosynthesis and degradation of lignin, response to environmental stresses such as wounding, pathogen attack and oxidative stress.
- 抗体英文名:
Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
- 抗体名:
HRP | Horseradish peroxidase (affinity purified)
- 规格:
1 mg
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文献和实验Conjugation of Antibodies to Horseradish Peroxidase
Horseradish peroxidase is coupled to IgG antibody in a two-step procedure. In the first step monosaccharide residues in the enzyme are oxidized with periodate to produce aldehyde groups. Then, in the second step, the aldehyde groups
Preparation of Horseradish Peroxidase-Labeled Probes
The direct labeling of nucleic acid probes with horseradish peroxidase (HRP) was first described by Renz at EMBL in 1984 (1 ). The methodology was combined with enhanced chemiluminescence (2 ) (a light producing HRP catalyzed reaction
Enhanced Chemiluminescent Detection of Horseradish Peroxidase Labeled Probes
Enhanced chemiluminescence (ECL) is a generic detection system that has been applied to all standard, membrane-based molecular biology techniques (1 ). The technology is based on the well-characterized horseradish peroxidase (HRP) catalyzed
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