- 询价
- Agrisera
- AS08 327
- 瑞典
- 2025年11月17日
- Immunofluorescence (IF), Western blot (WB)
- Rabbit
- Arabidopsis thaliana, Phaeodactylum tricornutum, Zea mays
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Sec21p | Gamma subunit, COP vesicles
- 抗体英文名：
Sec21p | Gamma subunit, COP vesicles
- 靶点：
Sec21p is a constituent of the COPI vesicle coatomer.
- 应用范围：
Immunofluorescence (IF), Western blot (WB)
- 宿主：
Rabbit
- 适应物种：
Arabidopsis thaliana, Phaeodactylum tricornutum, Zea mays
- 抗原来源：
Q0WW26
- 级别：
Serum
- 供应商：
Agrisera AB
- 克隆性：
单克隆
- 保存条件：
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the ca
- 形态：
Lyophilized
- 免疫原：
GST fusion of a part of recombinant Sec21 of Arabidopsis thaliana Q0WW26, At4g34450
- 规格：
50µl
| Immunogen: | GST fusion of a part of recombinant Sec21 of Arabidopsis thaliana Q0WW26, At4g34450 |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Purity: | Serum |
| Format: | Lyophilized |
| Quantity: | 50 µl |
| Reconstitution: | For reconstitution add 50 µl of sterile water |
| Storage: | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | Immunofluorescence (IF), Western blot (WB) |
| Recommended dilution: | 1 : 1000 (IF), 1 : 1000 (WB) |
| Expected | apparent MW: | 98 kDa |
Application example
50 µg of total protein from (1) Nicotiana tabacum protoplast total protein, (2) Arabidopsis thaliana protoplast soluble protein, (3) Arabidopsis thaliana protoplast total protein were separated on 10 % SDS-PAGE and blotted 2h to nitrocellulose (Semi-dry, 200mA). Filters were blocked over night with 5% low-fat milk powder in TBS and probed with anti-Sec21p antibodies (AS08 327, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP) in TBS-Tween. Signal was detected with chemiluminescent detection reagent, exposure time was 1 minute.
Protoplasts were extracted in 50mM Tris, 10 mM EDTA and Triton X100, 0.02%.
Immunofluorescence
Immunofluorescence labelling of rabbit anti-SEC21 (gamma subunit of COP vesicles; red) in 5-day-old root epidermal cells of Arabidopsis thaliana expressing ER-mGFP5-HDEL (ER marker; green). The antibody was diluted 1:1000 and the secondary antibody, donkey anti-rabbit CY5-coupled (Jackson ImmunoResearch) was diluted 1:300. The nuclei were stained with DAPI (blue).
Courtesy of Dr. Anna Gustavsson and Dr. Markus Grebe, Umeå Plant Science Centre, Sweden
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文献和实验| Selected references: | Hurny et al. (2020). SYNERGISTIC ON AUXIN AND CYTOKININ 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance. Nat Commun. 2020 May 1;11(1):2170. doi: 10.1038/s41467-020-15895-5. (immunolocalization) Lupette et al. (2019). The architecture of lipid droplets in the diatom Phaeodactylum tricornutum. Algal Research Volume 38, March 2019, 101415. Singh et al. (2018). A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis. PLoS Genet. 2018 Nov 15;14(11):e1007795. doi: 10.1371/journal.pgen.1007795. Kitakura et al. (2017). BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana. Plant Cell Physiol. 2017 Oct 1;58(10):1801-1811. doi: 10.1093/pcp/pcx118. Nagel et al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114. Wang et al. (2016). Comprehensive proteomic analysis of developing protein bodies in maize (Zea mays) endosperm provides novel insights into its biogenesis. J Exp Bot. 2016 Dec;67(22):6323-6335. Epub 2016 Oct 27. Wattelet-Boyer et al. (2016). Enrichment of hydroxylated C24- and C26-acyl- chain sphingolipids mediates PIN2 sorting at trans-Golgi network subdomains. Nat Commun. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788. Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314. Tanaka et al. (2013). Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis thaliana. PLoS Genet. May;9(5). (immunolocalization). Hopff et al. (2013). The plasma membrane proteome of maize roots grown under low and high iron conditions. J Proteomics Jan 24. Pimpl et al (2000). In situ localization and in vitro induction of plant COPI-coated vesicles. Plant Cell. 2000 Nov;12(11):2219-36. |
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
, macropain) activator subunit 2 (PA28 beta) (PSME2), mRNA 697 NM_002733 Homo sapiens protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1), mRNA 497 NM_002631 Homo sapiens phosphogluconate dehydrogenase (PGD), mRNA 1009 NM_002574 Homo
with the recommended amount of ATP, 2 μl of gamma-33P-ATP (20 μCi), and 2 μl of purified enzyme (10 units of cAMP-dependent protein kinase (catalytic subunit), 1000 units of casein kinase II, or 100 units of Erk2). Following the 1 hr incubation, the slides were washed
所有的看家基因(housekeeping genes)的列表+引物设计服务
gene encoding mitochondrial protein, mRNA 951 NM_005998 Homo sapiens chaperonin containing TCP1, subunit 3 (gamma) (CCT3), mRNA 1098 NM_005997 Homo sapiens transcription factor-like 1 (TCFL1), mRNA
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