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HSP101 | ClpB heat shock prote

in, C-terminal
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  • 询价
  • Agrisera
  • AS08 287
  • 瑞典
  • 2026年03月01日
  • Western blot (WB)
  • Rabbit
  • Arabidopsis thaliana, Agave tequilana, Citrus sp., Cucumis sativus L. var Krak
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      HSP101 | ClpB heat shock protein, C-terminal

    • 抗体英文名

      HSP101 | ClpB heat shock protein, C-terminal

    • 靶点

      Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family a

    • 应用范围

      Western blot (WB)

    • 宿主

      Rabbit

    • 适应物种

      Arabidopsis thaliana, Agave tequilana, Citrus sp., Cucumis sativus L. var Krak

    • 抗原来源

      P42730

    • 级别

      Serum

    • 供应商

      Agrisera AB

    • 克隆性

      单克隆

    • 保存条件

      Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the ca

    • 形态

      Lyophilized

    • 免疫原

      Recombinant protein of Arabidopsis thaliana Hsp101/ClpB, C-terminal 145 amino acids UniProt: P42730, TAIR: At1g74310

    • 规格

      50 µl

    When using this antibody on a new species with this antibody perform a following experiment: prepare a leaf extract from a leaf without stress and one that has been heat stressed as follows: Take a whole leaf (in the case of plants with small leaves, like Arabidopsis thaliana), or part of a leaf and place on wet filter paper in a petri dish. Heat stress in the dark at 38°C/90 minutes to 2 hours and then allow to recover for 2 hours at room temperature in low light (leave it on the lab bench). You should be able to load 10 ug for western blotting and compare the non-heat stressed to the heat stressed sample. Use Arabidopsis thaliana leaf as a positive control. Following such treatment 1 ug of a total protein from Arabidopsis thaliana allows detection of HSP101.

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