- 询价
- Agrisera
- AS05 096
- 瑞典
- 2025年11月04日
- Immunolocalization (IL), Western blot (WB)
- Rabbit
- Arabidopsis thaliana, Vicia faba
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
TOP2 | DNA topoisomerase II
- 抗体英文名：
TOP2 | DNA topoisomerase II
- 靶点：
Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomeras
- 应用范围：
Immunolocalization (IL), Western blot (WB)
- 宿主：
Rabbit
- 适应物种：
Arabidopsis thaliana, Vicia faba
- 抗原来源：
P30182
- 级别：
Serum
- 供应商：
Agrisera AB
- 克隆性：
单克隆
- 保存条件：
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the ca
- 形态：
Lyophilized
- 免疫原：
The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890, protein accesion number P30182) with an N-terminal hexahistidine tag was expressed in E.coli and purified by Ni2+ affinity chromatography.
- 规格：
200 µl
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文献和实验Topoisomerase I and II Activity Assays
DNA topoisomerases I and II (top1 and top2, respectively) are ubiquitous enzymes that play an essential role in transcription, replication, chromosome segregation, and DNA repair. The basic enzymatic reaction of topoisomerases
Assaying Topoisomerase II Checkpoints in Yeast
Topoisomerase II activity is crucial to maintain genome stability through the removal of catenanes in the DNA formed during DNA replication and scaffolding the mitotic chromosome. Perturbed Topo II activity causes defects in chromosome
DNA Topoisomerase II-Catalyzed DNA Decatenation
A major role of DNA topoisomerase II in vivo is to catalyze the double-stranded cleavage of DNA, allowing passage of a second DNA duplex through the break. This activity requires adenosine triphosphate (ATP) and is necessary for separating
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