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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
N-terminal 20 amino acids of aldolase C protein, MPHSYPALSAEQKKELSDIA
- 亚型:
Mouse monoclonal antibody to Aldolase C
- 形态:
Mouse monoclonal antibody to Aldolase C
- 保存条件:
厂家建议常温运输。冻干粉抗体溶解之后,建议分装保存于-20℃。请避免反复冻融。
- 克隆性:
单克隆
- 标记物:
Mouse monoclonal antibody to Aldolase C
- 适应物种:
Mouse monoclonal antibody to Aldolase C
- 保质期:
根据订购时间与产品批次判定,欢迎联系艾美捷咨询确认
- 抗原来源:
小鼠
- 目录编号:
Mouse monoclonal antibody to Aldolase C
- 级别:
一抗
- 库存:
小鼠来源毒性抗体:Aldolase C
- 供应商:
艾美捷
- 宿主:
小鼠
- 应用范围:
Mouse monoclonal antibody to Aldolase C
- 浓度:
Mouse monoclonal antibody to Aldolase C
- 靶点:
Mouse monoclonal antibody to Aldolase C
- 抗体英文名:
Mouse monoclonal antibody to Aldolase C
- 抗体名:
小鼠来源毒性抗体:Aldolase C
- 规格:
100ug
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文献和实验Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
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