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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Calpain-3 (CAPN3), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Calpain-3 (CAPN3), partial品名简称:
Recombinant Human CAPN3 protein, partial货号:
CSB-EP004497HU规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
CAPN3Uniprot No.:
P20807别名:
Calcium-activated neutral proteinase 3;CANP 3;Calpain L3;Calpain p94;Muscle-specific calcium-activated neutral protease 3;New calpain 1;nCL-1种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
46.8 kDa表达区域:
74-417aa氨基酸序列:
LYVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIDGANRTDICQGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVAEFFEIRDAPSDMYKIMKKAIERGSLMGCSIDDGTNMTYGTSPSGLNMGELIARMVRNMDNSLLQDSDLDPRGSDERPTRTIIPVQYETRMACGLVRGHAYSVTGLDEVPFKGEKVKLVRLRNPWGQVEWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLT蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Calcium-regulated non-lysosomal thiol-protease. Proteolytically cleaves CTBP1 at 'His-409'. Mediates, with UTP25, the proteasome-independent degradation of p53/TP53.功能2:
/文献:
/研究领域:
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文献和实验The methods described in this chapter are designed for rapid and convenient separation and quantitation of calpain isozymes in cultured osteoblastic cells by modern semiautomated strong-anion exchange FPLC. The isozymes of the calpain
The Role of Calpain in Neurofilament Protein Degradation Associated With Spinal Cord Injury
Calpain is a calcium-activated neutral proteinase present in all mammalian tissues thus far studied (1 ,2 ). Ubiquitous calpain exists as μ-calpain and m-calpain isoforms which require μM and mM calcium levels for activation
Studies of the Ubiquitin Proteasome System
( protocol 33 ), or mouse E1 ( protocol 34 ) 100 mM HEPES, pH 7.5 10× thiolester buffer (see recipe ) [32 P]ubiquitin ( protocol 26 ) or 10 µg/µl “cold
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