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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Chicken Glycine cleavage system H protein, mitochondrial (GCSH)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Chicken Glycine cleavage system H protein, mitochondrial (GCSH)品名简称:
Recombinant Chicken GCSH protein货号:
CSB-EP009335CH规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
GCSHUniprot No.:
P11183别名:
(Lipoic acid-containing protein)种属:
Gallus gallus (Chicken)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
18.7 kDa表达区域:
40-164aa氨基酸序列:
SARKFTDKHEWISVENGIGTVGISNFAQEALGDVVYCSLPEIGTKLNKDDEFGALESVKAASELYSPLTGEVTDINAALADNPGLVNKSCYQDGWLIKMTVEKPAELDELMSEDAYEKYIKSIED蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
The glycine cleavage system catalyzes the degradation of glycine. The H protein (GCSH) shuttles the methylamine group of glycine from the P protein (GLDC) to the T protein (GCST).功能2:
/文献:
"Chicken liver H-protein, a component of the glycine cleavage system. Amino acid sequence and identification of the N epsilon-lipoyllysine residue." Fujiwara K., Okamura-Ikeda K., Motokawa Y. J. Biol. Chem. 261:8836-8841(1986)研究领域:
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文献和实验James Hardwick CNBr Cleavage Procedure
in deionized H2O after the transfer is complete. This removes any residual Tris-glycine that seems to affect the migration of the CNBr fragments during SDS-PAGE. 2. Cut out the piece of NC containing the protein and immediately put it into a microfuge
vessel so they are upright. Include one vial containing a calibration protein. Assemble the hydrolysis vessel tightly. Evacuate the vessel for 10 sec (the acid should boil), and then flush with argon. Repeat the evacuation / flush steps. Finally
1: Measurement of Radiolabeled ATP Cleavage by Carboxylic Acid:CoA Ligase Alternate Protocol 2: Spectrophotometric Assay of Carboxylic Acid:CoA Ligase Activity using DTA Alternate Protocol 3: Indirect Assay
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