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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Lipoprotein lipase (Lpl)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4374.0 |
| 规格: | 20μg | 产品价格: | ¥2328.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
LplUniprot No.:
P11152别名:
LPL种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
57.3 kDa表达区域:
28-474aa氨基酸序列:
ADAGRDFSDIESKFALRTPEDTAEDTCHLIPGLADSVSNCHFNHSSKTFVVIHGWTVTGMYESWVPKLVAALYKREPDSNVIVVDWLYRAQQHYPVSAGYTKLVGNDVARFINWMEEEFNYPLDNVHLLGYSLGAHAAGVAGSLTNKKVNRITGLDPAGPNFEYAEAPSRLSPDDADFVDVLHTFTRGSPGRSIGIQKPVGHVDIYPNGGTFQPGCNIGEAIRVIAERGLGDVDQLVKCSHERSIHLFIDSLLNEENPSKAYRCNSKEAFEKGLCLSCRKNRCNNLGYEINKVRAKRSSKMYLKTRSQMPYKVFHYQVKIHFSGTEDGKQHNQAFEISLYGTVAESENIPFTLPEVSTNKTYSFLIYTEVDIGELLMMKLKWISDSYFSWPDWWSSPSFVIERIRVKAGETQKKVIFCAREKVSHLQKGKDSAVFVKCHDKSLKKSG蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
Signal TransductionReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1 plays a critical role in the lipolytic processing of chylomicrons." Beigneux A.P., Davies B.S.J., Gin P., Weinstein M.M., Farber E., Qiao X., Peale F., Bunting S., Walzem R.L., Wong J.S., Blaner W.S., Ding Z.-M., Melford K., Wongsiriroj N., Shu X., de Sauvage F., Ryan R.O., Fong L.G., Bensadoun A., Young S.G. Cell Metab. 5:279-291(2007)Function:
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文献和实验脂蛋白脂肪酶(lipoprteinlipase,LPL)是脂肪细胞、心肌细胞、骨骼肌细胞、乳腺细胞以及巨噬细胞等实质细胞合成和分泌的一种糖蛋白,分子量为60ku,含3%-8%碳水化合物。活性LPL以同源二聚体形式存在,通过静电引力与毛细血管内皮细胞表面的多聚糖结合,肝素可以促进此结合形式的LPL释放入血,并可提高其活性。LPL生理功能是催化CM和VLDL核心的TG分解为脂肪酸和单酸甘油酯,以供组织氧化供能和贮存。LPL还参与VLDL和HDL之间的载脂蛋白和磷脂的转换,ApoC
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠脂蛋白脂酶 ( LPL )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 LPL 单抗包被于酶标板上,标准品和样品中的 LPL与单抗结合,加入生物素化的抗小鼠 LPL
大鼠脂蛋白脂酶 (LpL) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定大鼠血清,血浆及相关液体样本 中 脂蛋白脂酶(LpL) 的 含量。 实验原理 : 本试剂盒应用双抗体夹心法测定 标本 中 大鼠 脂蛋白脂酶 (LpL) 水平。用纯化的 大鼠 脂蛋白脂酶 (LpL) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 脂蛋白脂酶 (LpL) , 再与HRP 标记的 脂蛋白
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