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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Pig Beta-defensin 1 (DEFB1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Pig Beta-defensin 1 (DEFB1)品名简称:
Recombinant Pig DEFB1 protein货号:
CSB-EP006662PI规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
DEFB1Uniprot No.:
O62697别名:
(BD-1)(Defensin, beta 1)种属:
Sus scrofa (Pig)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
31.1 kDa表达区域:
24-64aa氨基酸序列:
NIGNSVSCLRNKGVCMPGKCAPKMKQIGTCGMPQVKCCKRK蛋白标签:
N-terminal GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility.功能2:
/文献:
"Cloning and characterization of the gene for a new epithelial beta-defensin. Genomic structure, chromosomal localization, and evidence for its constitutive expression." Zhang G., Hiraiwa H., Yasue H., Wu H., Ross C.R., Troyer D., Blecha F. J. Biol. Chem. 274:24031-24037(1999)研究领域:
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文献和实验familar 小妹困惑,在此请教大师! 做了几次beta-catenin的表达(western),都没做出来,目的蛋白的分子量是92,可是,做出来的印记总是在下方,明显的不是目的蛋白的位置。我师兄说,做beta-catenin要提核蛋白才能做出来,可我之前也有做出来过的!请教各位有经验的同志,这样的现象是何解? woxingwosu 不一定要提核蛋白才能出来。b-catenin在核内核外都有分布。应该
lope 用WB检测beta-catenin的活性,应该是一种检测磷酸化水平,一种检测核内水平。 用前一种方法可以省下一个核内参,需要同时检测非磷酸化水平的吗? 而我看到的文献似乎还是检测核beta-catenin更多,请问前辈们是不是检测核蛋白更容易出结果呢? ylhuang0502 如检测磷酸化水平,LZ还需要检测总的catenin (磷酸化和非磷酸化都识别), 将p-beta
下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
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