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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified Mouse IgG Fc
- 形态:
Immunogen affinity purified goat IgG.
- 保存条件:
Store at 2 - 8 °C.
- 克隆性:
Polyclonal
- 适应物种:
Mouse IgG Fc, F(ab)’2 Fragment
- 供应商:
Agrisera
- 宿主:
Goat
- 应用范围:
ELISA (ELISA), interdigitated array (IDA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
- 靶点:
Goat anti-Mouse IgG Fc, F(ab)´2 Fragment
- 抗体英文名:
Goat anti-Mouse IgG Fc, F(ab)´2 Fragment
- 抗体名:
Goat anti-Mouse IgG Fc, F(ab)´2 Fragment
- 规格:
1.0 mg
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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
Acetone Mouse MAb to ganglioside Mouse MAb for control experiment FITC-conjugated goat F(ab')2 fragment to mouse IgM (µ chain-specific) FITC-conjugated goat F(ab')2 fragment to mouse IgG
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000 - 1:20,000 dilution in 4% BSA in TBST. This should be titrated for optimal results. I incubate with primary Ab 3 hours - overnight at room temp with shaking.10. Remove the antibody solution
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