- 询价
- Agrisera
- Sweden
- AS16 3437
- 2026年01月01日
- Immunoassays
- Donkey
- Mouse IgG (H&L)
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified Mouse IgG, whole molecule
- 形态：
Immunogen affinity purified donkey IgG.
- 保存条件：
Store at 2-8°C.
- 克隆性：
Polyclonal
- 适应物种：
Mouse IgG (H&L)
- 供应商：
Agrisera
- 宿主：
Donkey
- 应用范围：
Immunoassays
- 靶点：
Donkey anti-Mouse IgG (H&L), F(ab)'2 Fragment
- 抗体英文名：
Donkey anti-Mouse IgG (H&L), F(ab)'2 Fragment
- 抗体名：
Donkey anti-Mouse IgG (H&L), F(ab)'2 Fragment
- 规格：
1.0 mg
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
buffer (pH9.0) ~ 200 ml. Filter protein mixture Add protein mixture or culture supernatant containing Ab (adjust pH to 7.8 with Binding buffer ; red color) to the Protein A column. Mouse antibodies of the IgG1 subclass
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