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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
DYKDDDDK (binds to Sigma FLAG®)
- 形态:
Immunogen affinity purified in 100 mM Sodium Phosphate (pH 7.4), 100 mM NaCl, 50 mM Sucrose, 1.5% BSA and 0.01% Thimerosal.
- 保存条件:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
- 克隆性:
Monoclonal
- 供应商:
Agrisera
- 宿主:
Mouse
- 应用范围:
Flow cytometry (Flow cyt), cell-based assays, Microarrays (MA), and Microplate applications (MPl)
- 靶点:
Anti-DYKDDDDK (binds to Sigma FLAG®), SureLight® R-Phycoerythrin conjugated (Clone M2)
- 抗体英文名:
Anti-DYKDDDDK (binds to Sigma FLAG®), SureLight® R-Phycoerythrin conjugated (Clone M2)
- 抗体名:
Anti-DYKDDDDK (binds to Sigma FLAG®), SureLight® R-Phycoerythrin conjugated (Clone M2)
- 规格:
100 µg
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文献和实验Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
Signaling and Sigma-Aldrich). Anti-β-actin (A5441) was from Sigma-Aldrich; horseradish peroxidase-conjugated anti-mouse IgG (#NA931) and anti-rabbit IgG (#NA934) were from GE Healthcare. Detection of antibody reactivity was performed using Immobilon Western
Incubating the membrane with primary antibody, which binds to the protein of interest Washing to remove any unbound primary antibody Incubating the membrane with a conjugated secondary antibody, which binds the first antibody Washing
, and 2) fractionation over BlueSepharose, which binds Wnts with high selectivity. The purificationconsists of four chromatography steps: Blue Sepharose, immobilized metal affinity chromatography (IMAC), gel filtration,and heparin cation exchange
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