- 询价
- Agrisera
- Sweden
- AS15 2921
- 2025年12月22日
- Western blot (WB)
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
HA-tag: CYPYDVPDYASL
- 形态：
Immunogen affinity purified in 25 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.12 mM ZnCl2, pH 7.4. with 2 mM sodium azide.
- 保存条件：
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
- 克隆性：
Monoclonal
- 供应商：
Agrisera
- 宿主：
Mouse
- 应用范围：
Western blot (WB)
- 靶点：
Anti-HA, conjugated to Alkaline Phosphatase (Clone 16B12)
- 抗体英文名：
Anti-HA, conjugated to Alkaline Phosphatase (Clone 16B12)
- 抗体名：
Anti-HA, conjugated to Alkaline Phosphatase (Clone 16B12)
- 规格：
100 µg
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文献和实验Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
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