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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Neurocalcin-delta (NCALD)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Neurocalcin-delta (NCALD)品名简称:
Recombinant Human NCALD protein货号:
CSB-EP015510HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
NCALDUniprot No.:
P61601别名:
NCALD; Neurocalcin-delta种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
49.1 kDa表达区域:
1-193aa氨基酸序列:
GKQNSKLRPEVMQDLLESTDFTEHEIQEWYKGFLRDCPSGHLSMEEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISKAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNRDGKLSLEEFIRGAKSDPSIVRLLQCDPSSAGQF蛋白标签:
N-terminal GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions.功能2:
May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions.文献:
"Polymorphisms in the 3' UTR in the neurocalcin delta gene affect mRNA stability, and confer susceptibility to diabetic nephropathy." Kamiyama M., Kobayashi M., Araki S., Iida A., Tsunoda T., Kawai K., Imanishi M., Nomura M., Babazono T., Iwamoto Y., Kashiwagi A., Kaku K., Kawamori R., Ng D.P., Hansen T., Gaede P., Pedersen O., Nakamura Y., Maeda S. Hum. Genet. 122:397-407(2007)研究领域:
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文献和实验-Delta)对多种新冠病毒变异株具有广谱保护力。 新冠病毒 circRNA 疫苗研发示意图 一、首创环状RNA制备平台 作为近几年兴起的突破性医学技术,mRNA 疫苗的基本原理是通过脂纳米颗粒(LNP)将 mRNA 导入体内来表达抗原蛋白,以刺激机体产生特异性免疫反应。2019年底新冠肺炎疫情(COVID-19)暴发后,针对性的 mRNA 疫苗(ModernamRNA-1273; Pfizer/BioNTechBNT162b2)在多种疫苗类型中脱颖而出。mRNA 疫苗的修饰及递送技术均产生
(melting temperature),引物与模板形成双链的内部稳定性(internal stability, 用ΔG 值反映),形成引物二聚体(primer dimer)及发夹结构(duplex formation and hairpin)的能值,在错配位点( false priming site ) 的引发效率, 引物及产物的GC 含量(composition),等等。必要时还需对引物进行修饰,如增加限制性内切酶位点,引进突变等。根据有关参考资料[24],引物设计应注意如下要点
一、引物设计step by step 1、在NCBI上搜索到目的基因,找到该基因的mRNA,在CDS选项中,找到编码区所在位置,在下面的origin中,Copy该编码序列作为软件查询序列的候选对象。 2、用Primer Premier5搜索引物 ①打开Primer Premier5,点击File-New-DNA sequence, 出现输入序列窗口,Copy目的序列在输入框内(选择As),此窗口内,序列也可以直接翻译成蛋白。点击Primer,进入引物窗口。 ②此窗口可以链
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