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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见说明书
- 保质期:
见说明书
- 英文名:
Recombinant Rat GTP-binding nuclear protein Ran(Ran)
- 库存:
99
- 供应商:
武汉华美生物/CUSABIO
- 规格:
20ug/100ug/1mg
Ran蛋白|Recombinant Rat GTP-binding nuclear protein Ran(Ran)|大肠杆菌/毕赤酵母/哺乳动物细胞/杆状病毒-昆虫细胞/无细胞 五大表达系统
Uniprot ID:P62828
别名:Ran; GTP-binding nuclear protein Ran; GTPase Ran; Ras-like protein TC4; Ras-related nuclear protein
种属:Rattus norvegicus (Rat)
氨基酸序列:参见官网
微信ID: Cusabio01 电话: 027-87939808
详情请登录:www.cusabio.cn
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文献和实验Reconstitution of Nuclear Import in Permeabilized Cells
The trafficking of protein and RNA cargoes between the cytoplasm and the nucleus of eukaryotic cells, which is a major pathway involved in cell regulation, is mediated by nuclear transport sequences in the cargoes and by shuttling transport
【原创】Protein A Sepharose 之进化篇。。。
洗脱条件比较剧烈,最后收集的蛋白质很有可能变性,或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域:E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域,仅由5个结构域组成,每个域均可以和IgG的Fc段结合,但不同的域结合强度略有差异。因此洗脱条件不均一,而且经常需要较低的pH值。如果减少串联结构域的个数,并且采取同型结构域串联,就可以避免不同结构域与抗体Fc 段亲和性的差异,洗脱条件会温和而均一。为此,我们可以做一个试验,比较一下
Reprogramming a GFP Reporter Gene Subjects It to Complex Lentiviral Gene Regulation
export machinery. Relevant steps include the concerted action of several cis-acting RNA elements with the viral Rev-shuttle protein and several cellular components (Ran1/Exportin; Crm1). Based on detailed understanding of the molecular mechanisms guiding
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