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- 保存条件:
对于蓝色活细胞荧光探针CytoTell Blue产品,我们推荐使用常温进行运输。在您收到货物后,我们建议在低于-15℃温度下冷冻保存,要求储存环境干燥并避免光照。产品有效期为12个月。
- 保质期:
根据订购时间与产品批次判定,欢迎联系艾美捷咨询确认
- 英文名:
CytoTell Blue/CytoTell Blue/CytoTell Blue
- 库存:
CytoTell Blue/CytoTell Blue/CytoTell Blue
- 供应商:
艾美捷
- CAS号:
CytoTell Blue/CytoTell Blue/CytoTell Blue
- 规格:
2x500Tests
产品名称:蓝色活细胞荧光探针CytoTell Blue/蓝色活细胞荧光探针CytoTell Blue/蓝色活细胞荧光探针CytoTell Blue-CytoTell Blue/CytoTell Blue/CytoTell Blue
产品货号:AAT-22252-E
产品规格:2x500Tests
背景资料:提供的蓝色活细胞荧光探针CytoTell Blue产品为固体形式,我们建议将其溶解到DMSO溶液中使用或保存。
保存建议:蓝色活细胞荧光探针CytoTell Blue/蓝色活细胞荧光探针CytoTell Blue/蓝色活细胞荧光探针CytoTell Blue-CytoTell Blue/CytoTell Blue/CytoTell Blue对于蓝色活细胞荧光探针CytoTell Blue产品,我们推荐使用常温进行运输。在您收到货物后,我们建议在低于-15℃温度下冷冻保存,要求储存环境干燥并避免光照。产品有效期为12个月。
产品描述:Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations。 Among all the existing fluorescent dyes CFSE is the preferred cell proliferation indicator that is widely used for live cell analysis。 However, it is impossible to use CFSE and its fluorescein analogs for GFP-transfected cells or for the applications where a FITC-labeled antibody is used since CFSE and its fluorescein analogs have the excitation and emission spectra almost identical to GFP or FITC。 CytoTell dyes are well excited at major laser lines such as 405 nm, 488 nm or 633 nm with multicolor emissions。 CytoTell dyes have minimal cytotoxicity, and are used for the multicolor applications with either GFP cell lines or FITC-labeled antibodies since they have either excitation or emission spectra distinct from fluorescein。 CytoTell Blue is a blue fluorescent dye that stains cells evenly。 As cells divide, the dye is distributed equally between daughter cells that can be measured as successive halving of the fluorescence intensity of the dye。 Cells labeled with Cell CytoTell Blue may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers。 CytoTell Blue has a peak excitation of 405 nm and can be excited by the violet (405 nm) laser line。 It has a peak emission of 450 nm and can be detected with a 450/20 band pass filter (equivalent to Pacific Blue, or BD Horizon V450), making it compatible with applications that utilize GFP or FITC antibodies for multicolor cell analysis。
点击:蓝色活细胞荧光探针CytoTell Blue/蓝色活细胞荧光探针CytoTell Blue/蓝色活细胞荧光探针CytoTell Blue-CytoTell Blue/CytoTell Blue/CytoTell Blue查看更详细产品说明,更多应用、储存、价格、货期等信息请致电垂询AAT Bioquest 中国区域授权总代理艾美捷科技有限公司。更多特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看每日生物评论网站。
美国AAT Bioquest Inc。(前身是ABD Bioquest,Inc。)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括显色、荧光和生物发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学,免疫学,细胞生物学和分子生物学等领域。作为AAT Bioquest Inc。的中国区域代理,艾美捷科技为中国客户提供光谱学检测技术并应用于生化、生理代谢和细胞分析领域的产品,包括显色,荧光和发光技术等全系列解决方案。
艾美捷科技有限公司,通过提供完整的产品和服务体系、便利的客户关系和供应链管理,为生命科学研究工作者提供整体打包方案。我们的企业的核心价值观:“激情、敬业、学习、创新、合作、卓越”。激发我们内在的创造力,提供有竞争力的生物医学产品和服务,持续为客户创造最大价值是我们的企业使命。我们的企业愿景是成为受人尊重、世界一流的生物医学产品及服务供应商。艾美捷科技与国内外优秀的生物试剂供应商保持着密切的合作关系,目前已成为众多国际知名品牌的中国总代理或一级代理,主要包括:AmyJet、AAT Bioquest、Abnova、Agrisera、Biovision、Biosensis、Cayman Chemical、Caisson Labs、Cytoskeleton、CytoNiChe、Epigentek、Equitech-Bio、Hycult Biotech、Jackson、LifeSensors、ProSpec、Norgen Biotek、Mabtech、Matreya、iRegene、StressMarq、ImmunoReagents、SouthernBiotech、Origene、US Biological、Solis BioDyne等,可以在第一时间为用户提供最前沿的专业资讯、完备的产品及物流服务。艾美捷服务升级,全方位实验服务项目上线啦~
关键词:生化试剂,蓝色活细胞荧光探针CytoTell Blue/蓝色活细胞荧光探针CytoTell Blue/蓝色活细胞荧光探针CytoTell Blue,CytoTell Blue
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文献和实验Trypan blue will stain dead or dying cells. Viable cells are able to repell the dye and do not stain. Note: Trypan blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted
Trypan blue viability test Outline: Trypan blue will stain dead or dying cells. Viable cells are able to repell the dye and do not stain. Note : Trypan blue has a greater affinity for serum proteins than for cellular protein
Transformation of Electrocompetent E. coli with Blue/White selection
to a bacteria tube. Repeat for additional DNA samples and control DNA. Rotate at 37ºC x30 min. to allow the bacteria to recover. Meanwhile plate LB/Amp plates with IPTG + X-gal if blue/white selection of colonies will be performed
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