- ￥5600
- GeneTex
- 美国
- GTX00703
- 2025年07月11日
- WB, ICC/IF, IP, ELISA, IHC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide corresponding to N-terminus of human VRK1, MPRVKAAQAGRQSSAKRHL-C
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX00703
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IP, ELISA, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
VRK1
- 抗体英文名：
VRK1 antibody [5D1]
- 抗体名：
VRK1 抗体 [5D1]
- 规格：
100 μg
ICC/IF analysis of HeLa cells using GTX00703 VRK1 antibody [5D1].
Upper panel : Interphase cells
Lower panel : Metaphase cells
Dilution : 1:100
Fixation : 4% PFA
WB analysis of HeLa cell lysate using GTX00703 VRK1 antibody [5D1].
Samples :
Lane 1 : HeLa cells transfected Luciferase RNAi (control)
Lane 2 : HeLa cells transfected VRK1-1 RNAi
Lane 3 : HeLa cells transfected VRK1-2 RNAi
Dilution : 1:500
WB analysis of various samples using GTX00703 VRK1 antibody [5D1].
Lane 1-3 : HeLa whole cell lysate
Lane 4-6 : U2OS whole cell lysate
Dilution :
Lane 1, 4 : 1:100
Lane 2, 5 : 1:500
Lane 3, 6 : 1:1000
Cell amount : 5 x10⁴ cells
ICC/IF analysis of U2OS cells using GTX00703 VRK1 antibody [5D1].
Upper panel : Interphase cells
Middle and lower panel : Metaphase cells
Dilution : 1:100
Fixation : Methanol
ICC/IF analysis of HeLa cells using GTX00703 VRK1 antibody [5D1].
Upper panel : Interphase cells
Middle and lower panel : Metaphase cells
Dilution : 1:100
Fixation : Methanol
WB analysis of TIG-1 cell lysate using GTX00703 VRK1 antibody [5D1].
Samples :
Lane 1 : TIG-1 cells transfected Luciferase RNAi (control)
Lane 2 : TIG-1 cells transfected VRK1-1 RNAi
Lane 3 : TIG-1 cells transfected VRK1-2 RNAi
Dilution : 1:500
ICC/IF analysis of U2OS cells using GTX00703 VRK1 antibody [5D1].
Upper panel : Interphase cells
Middle and lower panel : Metaphase cells
Dilution : 1:100
Fixation : 4% PFA
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文献和实验Immunodetection of cyclin D1 and D2/D3 using flow cytometry
. It has been tested with antibodies against cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326), cyclin D2/D3 (cat. no. 14711A, clone G107-22) and cyclin E Procedure Prepare the cell type of interest as a single cell suspension and wash once with cold PBS
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
. It has been tested with antibodies against cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326), cyclin D2/D3 (cat. no. 14711A, clone G107-22) and cyclin E, and is based on protocols originally designed by Z. Darzynkiewicz and colleagues. Although FITC-conjugated
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry
Immunodetection of cyclin D1 and D2 /D3 using 488/630 nm dual laser flow cytometry William Telford Hospital for Special Surgery
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