- ￥5600
- GeneTex
- 美国
- GTX00695
- 2025年07月13日
- WB, ICC/IF
- Mouse
- Human, Mouse, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Tetrahymena
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptides containing conserved N-terminal sequence, GLFG, of Nup98 protein of Tetrahymena thermophila. <br>Peptide 1; 1-MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ-29 <br>Peptide 2; 646-SNPTQGGGLFGAANPGLGG-664 <br>Epitope determined: GLF
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Tetrahymena
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Tetrahymena
- 目录编号：
GTX00695
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
For WB application, it works on S. cerevisiae and S. pombe nut may be not suitbale for human, T. etrahymena and yeasts. Recommend GTX00693 NUP98 antibody [13C2] on human, T. etrahymena and yeasts samples.
- 抗体英文名：
NUP98 antibody [21A10]
- 抗体名：
NUP98 抗体 [21A10]
- 规格：
100 μg
WB analysis of S. cereviciae celll extracts using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Dilution :
13C2 or 21A10 : 1:10
2H10 : 20 μg/ml
WB analysis of Tetrahymena themophila cells or Tetrahymena themophila cells overexpressing GFP-MacNup98A using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Diamonds and asterisks represent uncharacterized proteins.
WB analysis of HeLa whole cell lysate using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Dilution : 0.4 μg/ml
Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.
ICC/IF analysis of S. pombe cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Green : Primary antibody
Violet : DAPI
Fixation : 4% PFA for 10 min, treated with 0.6 mg/ml Zymolyase 100T at 3 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min
ICC/IF analysis of Tetrahymena themophila cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. 13C2 mAb was highly specific to the macronucleus. In contrast, in addition to clear macronuclear staining, 21A10 mAb also stained the micronuclear periphery. This indicates that 21A10 mAb recognizes Nups localizing to the micronucleus such as Nup308 in addition to MacNup98A. Neither mABs 2H10 nor 414 could stain nuclear periphery of Tetrahymena.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min
WB analysis of S. pombe cell l extracts using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. Left and right lanes represent specimens from a wild type strain and an S. pombe strain in which Nup98 was chromosomally replaced with Nup98-GFP, respectively.
Dilution :
13C2 or 21A10 : 1:10
2H10 : 1 μg/ml
ICC/IF analysis of HeLa cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The signal at the nuclear periphery with 21A10 mAb was much higher and the background lower than that of 2H10 and 13C2 antibodies.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min
ICC/IF analysis of S. cereviciae cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Green : Primary antibody
Violet : DAPI
Dilution :
13C2 or 21A10 : 1:10
2H10 : 10 μg/ml
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文献和实验Iwamoto M et al., Monoclon Antib Immunodiagn Immunother. 2013 (PMID:23607342)
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