Recombinant human ATF6 alpha; (His-tagged amino-terminal fragment of ATF6 alpha)
亚型:
IgG2a
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Monoclonal
标记物:
Unconjugated
适应物种:
Human
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX00687
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Mouse
应用范围:
WB, ICC/IF, IP
浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
This antibody detects both full-length ATF6 alpha as well as the cleaved ATF6 alpha p50 form.
抗体英文名:
ATF6 antibody [1-7]
抗体名:
ATF6 抗体 [1-7]
规格:
50 μg
ICC/IF analysis of HeLa cells using GTX00687 ATF6 antibody [1-7]. Green : Primary antibody Blue : DAPI Dilution : 1:100
IP analysis of HeLa or 293 cell lysates using GTX00687 ATF6 antibody [1-7]. It is recommended using a light chain specific anti-mouse IgG antibody as a secondary antibody. Lane 1, 2, 7, and 8 : Input Lanes 3, 4, 9, and 10 : Non-related IgG as IP antibody Lanes 5, 6, 11, and 12 : GTX00687 as IP antibody IB antibody: GTX00687 ATF6 antibody [1-7]
WB analysis of various samples using GTX00687 ATF6 antibody [1-7]. The asterisk denotes an unglycosylated form of pATF6α(P). ATF6α is constitutively expressed as pATF6α(P), and converted to pATF6α(N) ( > 50-kDa protein) in ER-stressed cells. pATF6α(P) : the endogenous precursor ATF6 alpha (~90kDa) pATF6α(N) : the cleaved product of ATF6 alpha (>50kDa) Tm: cells treated with 2 μg/ml tunicamycin (inhibitor of N-glycosylation)< br>DTT: cells treated with 1mM dithiothreitol (reducing reagent). Loading : 50μg