Recombinant ATF6 alpha; (amino-terminal fragment of ATF6 alpha; fused to GST)
亚型:
IgG1
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Monoclonal
标记物:
Unconjugated
适应物种:
Human
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX00686
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Mouse
应用范围:
WB, IP
浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
This antibody detects both full-length ATF6 alpha as well as the cleaved ATF6 alpha p50 form.This antibody is less efficient for IP compared to GTX00687,We do not recommend use of this product for Mouse.
抗体英文名:
ATF6 antibody [37-1]
抗体名:
ATF6 抗体 [37-1]
规格:
100 μg
WB analysis of HeLa cell lysate using GTX00686 ATF6 antibody [37-1]. The asterisk denotes an unglycosylated form of pATF6α(P). ATF6α is constitutively expressed as pATF6α(P), and converted to pATF6α(N) ( > 50-kDa protein) in ER-stressed cells. pATF6α(P) : the endogenous precursor ATF6α (~90kDa) pATF6α(N) : the cleaved product of ATF6α(>50kDa) Lane 1 : untreated Lane 2 : 1mM DTT (reducing reagent) for 1 h Lane 3 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 3 h Lane 4 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 7 h. Loading : 50μg
WB analysis of NIH-3T3 cell lysate using GTX00686 ATF6 antibody [37-1]. The asterisk denotes an unglycosylated form of pATF6α(P). ATF6α is constitutively expressed as pATF6α(P), and converted to pATF6α(N) ( > 50-kDa protein) in ER-stressed cells. pATF6α(P) : the endogenous precursor ATF6α (~90kDa) pATF6α(N) : the cleaved product of ATF6α(>50kDa) Lane 1 : untreated Lane 2 : 1mM DTT (reducing reagent) for 1 h Lane 3 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 3 h Lane 4 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 7 h. Loading : 50μg