- ￥6500
- GeneTex
- 美国
- GTX00686
- 2025年07月15日
- WB, IP
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant ATF6 alpha; (amino-terminal fragment of ATF6 alpha; fused to GST)
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX00686
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, IP
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody detects both full-length ATF6 alpha as well as the cleaved ATF6 alpha p50 form.This antibody is less efficient for IP compared to GTX00687,We do not recommend use of this product for Mouse.
- 抗体英文名：
ATF6 antibody [37-1]
- 抗体名：
ATF6 抗体 [37-1]
- 规格：
100 μg
WB analysis of HeLa cell lysate using GTX00686 ATF6 antibody [37-1]. The asterisk denotes an unglycosylated form of pATF6α(P). ATF6α is constitutively expressed as pATF6α(P), and converted to pATF6α(N) ( > 50-kDa protein) in ER-stressed cells.
pATF6α(P) : the endogenous precursor ATF6α (~90kDa)
pATF6α(N) : the cleaved product of ATF6α(>50kDa)
Lane 1 : untreated
Lane 2 : 1mM DTT (reducing reagent) for 1 h
Lane 3 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 3 h
Lane 4 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 7 h.
Loading : 50μg
WB analysis of NIH-3T3 cell lysate using GTX00686 ATF6 antibody [37-1]. The asterisk denotes an unglycosylated form of pATF6α(P). ATF6α is constitutively expressed as pATF6α(P), and converted to pATF6α(N) ( > 50-kDa protein) in ER-stressed cells.
pATF6α(P) : the endogenous precursor ATF6α (~90kDa)
pATF6α(N) : the cleaved product of ATF6α(>50kDa)
Lane 1 : untreated
Lane 2 : 1mM DTT (reducing reagent) for 1 h
Lane 3 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 3 h
Lane 4 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 7 h.
Loading : 50μg
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文献和实验Mori K et al., Mol Biol Cell. 2010 (PMID:20219975)
to 0.05, and zymolyase (100T) to 60 ug/ml. Incubate 1 hour at 37oC. Pellet spheroplasts as above. Resuspend in 10 mls Solution A and pellet again. Combine the suspension of whole cells with the spheroplasts and pellet as above. Resuspend in 10
IMMUNOSCREENING OF WITH ANTIBODY PROBES
Adsroption of Anti-E. coli Antibodies Grow E. coli strain Y1090 in L broth at 37°C overnight. Transfer to 10 mM MgSO4 and store at 4°C for future use. These cells are good for about 1 week. Soak one 132 mm nitrocellulose membrane per Petri dish
Antibody cleanup In order to decrease the amount of nonspecific staining, it is often necessary to preabsorb primary and secondary antibodies to yeast cells lacking the antigen prior to use. A 1:1 mixture of fixed yeast
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