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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Pericentrin (Pcnt), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Pericentrin (Pcnt), partial品名简称:
Recombinant Mouse Pcnt protein, partial货号:
CSB-EP017627MO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP017627MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Pcnt.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
PcntUniprot No.:
P48725别名:
Pcnt2种属:
Mus musculus (Mouse)蛋白长度:
Partial来源:
E.coli分子量:
37.7 kDa表达区域:
2545-2810aa氨基酸序列:
LCAAGLLTSFTNHTVDRTIKDWTSSNEKAVSSLMRTLEELKSELSMPTSFQKKMTAELQVQLMNELLSDNDALTKAVGMATREKAELCRTVSRLEKTLKHHTQKGCVLNRQSKSSLKQDGTDLQSSLRHSDPEWHSQTTSGDTNTCNIKMEKLYLHYLRAESFRKALIYQKKYLLLLIGGFQDSEQETLSMIAHLGVFPSKADKKITMSRPFTKFRTAVRVVIAVLRLRFLVKKWQEVDRKGALVHPKSTRHGHRTSQRQRSPSGP蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material. May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.功能2:
/文献:
"LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome." Feng Y., Olson E.C., Stukenberg P.T., Flanagan L.A., Kirschner M.W., Walsh C.A. Neuron 28:665-679(2000)研究领域:
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文献和实验071794 h 货号:CSB-RP071794(A4/C) 兔抗人淀粉样肽 beta A4 蛋白多克隆抗体 货号:CSB-PA07179A0Rb 识别种属:人,小鼠 WB:Mouse heart tissue,Mouse kidney tissue,Mouse lung tissue,293,A549,U251,SH-SY5Y IHC:human kidney
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31.25-2000pg/ml EU3598 MSTN ELISA Kit 18.75pg/ml 31.25-2000pg/ml 重组蛋白&抗体 货号 产品名称 宿主 分子量/应用 P0302 Recombinant Human GDF-8 E.Coli 33.3 kDa Pr22272 Recombinant Mouse Latent
实验助攻 | WB 实验 (Western Blot) 杂带太多?原因就这几个!
发生降解时,完整的蛋白分子会被蛋白酶切断。如果降解发生在抗体结合域,或者不同分子被切割的位置不同,就会产生分子量比原目的蛋白小的一系列截短片段。此时,WB 条带中不仅会有原本的主带,还会出现多条低分子量的杂带或条带模糊、向下拖尾的现象。 Tips 如果使用纯化的重组蛋白作为阳性对照只有单条带,而你的实验样本出现多条带,则降解可能性极大。 【建议】 ① 添加抑制剂:裂解液确保含有蛋白酶抑制剂,并超声,抑制内源性蛋白酶的活性。 ② 低温操作:样本收集、处理和裂解的全过程必须在冰上进行,并使用
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