小鼠Wee1重组蛋白(Wee1A kinase)Mouse

英文名称：

Recombinant Mouse Wee1-like protein kinase (Wee1)

品名简称：

Recombinant Mouse Wee1 protein

货号：

CSB-EP026084MO

规格:

20μg

价格：

2328

LC-MS/Western Blot：

/

SDS-PAGE：

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

纯度：

Greater than 85% as determined by SDS-PAGE.

内毒素：

Not test

生物活性：

/

基因名：

Wee1

Uniprot No.：

P47810

别名：

(Wee1A kinase)

种属：

Mus musculus (Mouse)

蛋白长度：

Full Length

来源：

E.coli

分子量：

77.5 kDa

表达区域：

1-646aa

氨基酸序列：

MSFLSRQQPPPTRRVGAAYSLRQKLIFSPGSDCEEEEEEEEEGSGHSTGEDSAFQEPDSPLPSARSPAEAEAERRRRSPGAEPSSPGELEDDLLLQGGGGGAQAAGGGAEGDSWEEEGFGSSSPVKSPSTAYFLSSPFSPVRCGGPGDASPQGCGAPRAMDDPCSPQPDYPSTPPHKTFRKLRLFDTPHTPKSLLSKARVIDSGSVKLRGSSLFMDTEKSGKREFDTRQTPQVNINPFTPDPVLLHSSGRCRGRKRAYFNDSSEDMEASDYEFEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHPHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRVMSYLTEVELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAVSEEGDEDDWISNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYSHLPKADIFALALTVVCAAGAEPLPRNGEQWHEIRQGRLPRIPQVLSQEVTELLRVMIHPDPERRPSAMELVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAAKVAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY

蛋白标签：

N-terminal 6xHis-tagged

产品提供形式：

Liquid or Lyophilized powder

缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

储存条件：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

货期：

13-23 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能1：

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

功能2：

/

文献：

"Mouse Wee1 gene is repressed by Kruppel-like factor 3 (KLF3) via interaction with multiple upstream elements." Kitamura T., Suzuki H., Tamura T.A. Gene 492:361-367(2012)

研究领域：

Cell Biology

请点击“在线询价”找客服索取产品说明书及引用文献目录，产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网