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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Ribonuclease 4 (RNASE4)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥17754.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4164.0 |
| 规格: | 20μg | 产品价格: | ¥2208.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
RNASE4Uniprot No.:
P34096别名:
RNS4种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
Yeast分子量:
29.8 kDa表达区域:
29-147aa氨基酸序列:
QDGMYQRFLRQHVHPEETGGSDRYCNLMMQRRKMTLYHCKRFNTFIHEDIWNIRSICSTTNIQCKNGKMNCHEGVVKVTDCRDTG_S_SRAPNCRYRAIASTRRVVIACEGNPQVPVHFDG蛋白标签:
N-terminal 6xHis-sumostar-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
This RNase has marked specificity towards the 3' side of uridine nucleotides.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
CardiovascularReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Human ribonuclease 4 (RNase 4): coding sequence, chromosomal localization and identification of two distinct transcripts in human somatic tissues." Rosenberg H.F., Dyer K.D. Nucleic Acids Res. 23:4290-4295(1995)Function:
This RNase has marked specificity towards the 3' side of uridine nucleotides.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏蛋白
肝炎病毒包膜E2蛋白相同的亲和结合力3。另外,有阴离子转运载体AE1结构域的重组蛋白被标记且用Rho1D4系统纯化后表现出与红细胞来源的AE1蛋白有相同的硫酸外排速率。4) 利用Rho1D4纯化膜蛋白图解简述步骤1:结合:将Rho1D4标记的蛋白与载有Rho1D4抗体的琼脂糖一同孵育使得目标蛋白被固定在吸附材料上。 步骤2:清洗:将杂蛋白和其他裂解液成分洗去,留下与亲和基质结合的目标蛋白。 步骤3:洗脱:过量Rho1D4肽与基质竞争性结合,释放出目标蛋白在洗脱液中收集。 表
100-18B Human FGF-basic,10mM Tri(pH 8.0)用于溶解100-25 Human FGF-8。 关于PeproTech公司重组细胞因子和蛋白溶解的具体方法和注意事项请见:http://www.biomart.cn/experiment/430/488/490/492/31321.htm PeproTech公司简介: PeproTech Inc. 创立于1988年,始终致力于为生命科学研究开发重组蛋白及相关产品
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