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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Thrombospondin-1 (Thbs1), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14844.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥3456.0 |
| 规格: | 20μg | 产品价格: | ¥1836.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
Thbs1Uniprot No.:
Q80YQ1别名:
/种属:
Mus musculus (Mouse)蛋白长度:
Partial来源:
Yeast分子量:
38.6 kDa表达区域:
19-350aa氨基酸序列:
NRIPESGGDNGVFDIFELIGGARRGPGRRLVKGQDLSSPAFRIENANLIPAVPDDKFQDLLDAVWADKGFIFLASLRQMKKTRGTLLAVERKDNTGQIFSVVSNGKAGTLDLSLSLPGKQQVVSVEEALLATGQWKSITLFVQEDRAQLYIDCDKMESAELDVPIQSIFTRDLASVARLRVAKGDVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSATNVLLTLDNNVVNG_S_SPAIRTNYIGHKTKDLQAICGLSCDELSSMVLELKGLRTIVTTLQDSIRKVTEENRELVSELKRPPLCFHNGVQYKNNEEWTVDSCTECHCQNSVTICK蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties . Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors.1 Publication内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
Characterization of the murine thrombospondin gene.Lawler J., Duquette M., Ferro P., Copeland N.G., Gilbert D.J., Jenkins N.A.Genomics 11:587-600(1991)Function:
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文献和实验V294.PART II,Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
macrophages to colony-stimulating factor-1 (CSF-1; ref. 8), fibroblaststo platelet-derived growth factor (7) and thrombospondin (TSP1) (9), neutrophilsto interleukin-8 (10), and microglia to adenosine triphosphate/adenosinediphosphate (11). In addition
最近的一些分子生物学进展使得一些生物技术工具极大提高了生物发光和化学发光的检测和快速应用。这些发展方便了体外和体内持续检测生物过程(如基因表达,蛋白质-蛋白质相互间作用和疾病的进程),可应用于临床、诊断、和药物开发等。而且,结合发光酶或某些在基因水平有生物特异结合位点的发光蛋白发展了超敏感和选择性的生物分析工具,如重组细胞生物传感器,免疫分析和核酸杂交系统。发光分析信号的高度可侦测性使得它非常适合于微小化的生物分析装置(如微矩阵,微流设备和高密度的微孔板)以用于小量样品体积的基因和蛋白的高通
了靶蛋白的水平。15,16 这个载体系统提高了将 shRNA导入多种组织细胞的效率。17 不过,由于腺病毒载体在细胞内保持游离,并且随着细胞的分裂而稀释,所以shRNA的表达是短暂的。腺病毒蛋白的低水平表达,以及对腺病毒蛋白的预存免疫也导致了表达shRNA的细胞数目的减少,限制了它在体内的应用。 腺相关病毒(AAV)载体系统很有应用前景,既能转染分裂细胞,又能转染非分裂细胞,而且不会致病,不会激发免疫反应。它可以以游离的形式在转染细胞长期存在,偶尔可以整合到宿主基因组里,使导入的基因长期表达
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